Proteomics Profilings Uncover Fusarium Head Blight Resistance Related Players In Wheat

Fusarium head blight(FHB)is a destructive disease of wheat that reduces yield and grain quality.High-throughput proteomic techniques have been used to identify a wide range of candidate proteins involved in host plant resistance to different diseases.The majority of the published works on the proteomics of the wheat responses to Fusarium graminearum infection are case specific studiesIn the current study,a high-throughput quantitative label-free strategy was employed on bulked rachides of F.graminearum-infected wheat collected from multiple genotypes Differentially accumulated proteins among the following four pools were identified:mock-inoculated FHB-resistant accessions(RC),mock-inoculated FHB-susceptible accessions(SC),F.graminearum-inoculated FHB-resistant accessions(RF),and F.graminearum-inoculated FHB-susceptible accessions(SF).A total of 126 differentially accumulated proteins was identified in RF vs.RC after comparative proteomics,133 proteins between SF vs.SC,115 proteins between RF vs.SF.Proteins were projected onto 17 consensus intervals of previously reported quantitative trait locus(QTL)in the FHB-resistant pool by blasting against the Chinese Spring reference sequences,a total of 404 proteins fell into different intervals,of which 38 were differentially accumulated among different comparisons,QTL matches leads to identifying candidates for certain QTL regions.Weighted correlation network analysis(WGCNA)was used to catch hub proteins that may relate to the host phenotype in response to F.graminearum.WGCNA constructs 23 different expression modules,34 new candidates in blue module have emerged in association with resistance or susceptibility,and 49 proteins in turquoise module which are related to the general FHB responseAmong the F.graminearum proteins identified,two are predicted to play key role in protein-protein interaction networks:ATP-dependent RNA helicase FAL1 and Elongation factor 1-alphaHigh-throughput proteomics was employed for pooled wheat rachides in the current study indicates the first study of protein atlas for FHB,providing new perspectives on the interaction between F.graminearum and wheat.