| In recent years,some studies have found that overnutrition or undernutrition can affect the animal's estrus rate,ovulation rate,conception rate,lambing rate and reproductive rate.However,Short-term flushing effects on the reproductive performance of ewes was unclear in our nation Therefore,this experiment was conducted to study the effects of short-term supplementation of soybean meal or corn on serum biochemical parameters and reproductive performance of Hu ewes before oestrus of ewes,and further to determine serum metabolomics for mechanism.In this experiment,120 Hu ewes at 3 years old with 3 parity were selected and divided into two groups according to the body condition score.One group was above 3 points(48.42 ± 3.69 kg),and the other group was below 3 points(40.47 ± 4.35 kg).Each group was randomly divided into 3subgroups,of which 1 subgroup was the control,and the other 2 subgroups were respectively supplementation of the corn or soybean meal.At the beginning and end of the feeding trial,the body weight was recorded.Ten ewes in one subgroup were selected from each subgroup for blood collection for determining serum hormones,growth factors,metabolic hormones and serum biochemical indicators.Based on the results of serum analysis,serum metabolomics analysis was performed on groups with significant differences.The results were as follows:(1)When the corn or soybean meal was supplemented from 1 to 14 days before the ewes were mated,there was no significant effect on the weight of the Hu ewes.(2)When supplemented with soybean meal,the serum GLU concentration of the soybean meal group with body score above and below 3 points was significantly higher than that of the control group on the 7th day(P<0.05);The TP concentration in the test group was on the 14 th and16th day significantly lower than that in the control group(P<0.05);The serum BUN concentration in the test group was significantly higher than that in the control group at each time point(P<0.05);Serum insulin and leptin concentrations in groups above 3 scores were significantly lower than those in the control group at the 14 th and 16 th day(on oestrus),7th and16 th day(on oestrus)(P<0.05).(3)When supplemented corn feed,the reproductive hormones,such as FSH,LH,and E2 concentration,were significantly higher than the control group below 3 points(P<0.05)at 16 d(on estrus).but there was no significant difference in the P4 concentration in the corn groups,The GLU concentration was significantly higher than that of the control group on the 7th day(P<0.05);the BUN concentration in the corn group above or below 3 score was significantly lower than that of the corresponding control group on the 0th and 7th day(P<0.05);Serum TP concentrations in the corn group above or below 3 points were significantly higher than those in the corresponding control group on the 7th and 14 th day(P<0.05).Serum insulin concentration in groups above 3scores,and leptin concentration in group with corn supplementation were significantly higher than those in the corresponding control group at the 16 th day(on oestrus)(P<0.05).(4)Single lambing rate in group above 3 points with supplementation of corn was higher than the corresponding control group and group supplementation of soybean meal(P<0.05),but which had higher twin lambing rates than group with supplementation of corn(P<0.05).Lambing rate below 3 points with supplementation of soybean meal and with supplementation of corn significantly higher than corresponding control group(P<0.05).(5)Metabolomics results showed that soybean meal supplemented in diet mainly affected the metabolism of amino acids,sugars,organic acids,amino acids and their derivatives;when supplemented corn in diet,it mainly impacted sugars and carboxylic acids.In conclusion,supplementing 250 g of soybean meal or corn with body condition below 3points before 1 to 14 days of mating can improve the reproductive performance of the Hu ewes. |
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