The Research Of Pharmacokinetics And Toxicology Of Sulfamethoxazole In Tilapia(GIFT Oreochromis Niloticus)

In order to understand the pharmacokinetics and toxicity of sulfamethoxazole(SMZ)in tilapia(Gift Oreochromis niloticus),the author take methods of continuous oral administration and single injection of drugs,and detected the concentrations changes of drugs in fish tissue and organ,results show that:1.By feeding SMZ bait 2 times a day,for 5 days,after first administration,the drug max concentration in liver for 4 hours,blood and muscles for 6 h,the second peak,respectively,followed by 2 h,4 h,8 h,after the last dose of the 168 h,blood,muscle of SMZ concentration had been lower than the minimum detection limit,only the liver had a small amount of residual concentration of 0.14±0.02 ?g/g.2.The injection of SMZ in plasma and liver were consistent with the open two compartment model.The pharmacokinetic parameters of plasma were: AUC 7286.38 mg/L·h,Cmax 760.53 mg/L,T1/2? 3.623 h,T1/2? 28.319 h,The main pharmacokinetic parameters of liver were: AUC 2926.09 mg/L·h,Cmax 7372.98 mg/L,T1/2? 1.468 h,T1/2? 26.144 h.3.After injection of 60 mg/kg weight dose of SMZ,the content of MDA and the activity of liver ERND,SOD injection of SMZ,GSH-Px and GST reached the maximum value at 24 h,and in 168 h were restored to normal level.And SMZ on EROD showed a certain inhibitory effect,3 h EROD activity dropped to the lowest value.In order to understand the use of drug bait interval,the study usd a single feeding mode,regularly checked the chyme process.The results showed that the changes of the instantaneous gastric contents(wet weight)with time showed a linear correlation: y =-0.2894 x + 4.6435,R2 = 0.9912.The gastric emptying time was 16 h,and the intestinal emptying was 28 h.