Protect Effects Of Low Molecular Seleno-aminopolysaccharide On Intestinal Mucosal Barrier In Weaning Stressed Animals

Early weaning can cause stress,which can easily cause damage to the intestinal mucosa,diarrhea,severe intestinal inflammation and other problems.Therefore,how to ensure and maintain the normal barrier function of intestinal mucosa has become a research hotspot.Based on the preliminary experiments,this paper used the intestinal mucosal injury test of diarrhea piglets and intestinal mucosal injury in weaning stress rats to investigated the protective effect of LSA on intestinal mucous barrier in weaning stressed animals and the regulatory effect and possible mechanism of LSA on MUC2 structure and function.By studying the morphology and function of intestinal mucosa,intestinal mucus thickness,the levels of anti-oxidation in intestinal mucosal,the expression levels of mucosal genes(MUC2,GalNacT1,GalNacT2 and GalNacT7),antioxidant genes and intestinal mucosal nuclear transcription factor Nrf2,NF-?B signaling pathway and its related factors in the path,we determine the protective effect of LSA on the intestinal mucus barrier of weaned stress animals.The results showed that early weaning would hinder the growth of young rats.Weaning damaged the intestinal mucosal barrier.Weaning increased intestinal damage and permeability in young rats.Weaning significantly reduced the mRNA expression levels of glycosyltransferases T1,T2,T7 and MUC2 of intestinal mucosal.In addition,weaning significantly reduced intestinal mucosal thickness and decreased number of goblet cells.Weaning significantly reduced the antioxidant function of weaned rats and increases the oxidative stress produced by weaning.Western Blot results showed that weaning significantly inhibited the protein expression level of Nrf2 and up-regulated the protein expression levels of Keap1.These results indicated that weaning activated Nrf2 signaling pathways of intestinal mucosa,prevented the transcription of Nrf2 into nuclear,reduced the expression of the downstream enzymes.In this experiment,we aimed to study on the effect of diarrhea piglets caused by K88 on structure and function of intestinal mucin MUC2.The results indicated that diarrhea caused the structural change of MUC2 and disrupted the intestinal mucosal barrier of piglets.We studied on the effects of Low Molecular Seleno-aminopolysaccharide on intestinal mucosal barrier in weaning rats.The results showed that LSA increased the growth performance of weaned rats.LSA had a protective effect on intestinal mucosal barrier damaged by weaning.LSA reduced intestinal damage and permeability in weaned rats.Furthermore,LSA can increase the number of MUC2 glycans and extend the length of MUC2 glycans.LSA can increase the acid glycans in the mucin MUC2 structure,indicating that LSA can reverse the changes of intestinal mucus protein structure caused by weaning.LSA significantly increased the mRNA expression levels of intestinal mucosal glycosyltransferases T1,T2,T7 and MUC2.In addition,LSA significantly increased intestinal mucosal thickness and number of goblet cells.LSA significantly increased the antioxidant function of weaned rats and reduced the oxidative stress produced by weaning.Moreover,LSA significantly increased the gene expression levels of GSH-Px,SOD and Nrf2,HO-1,suggesting that LSA may protect the intestinal mucus barrier of weaned stress animals by activating Nrf2 pathway.Western Blot results showed that LSA significantly up-regulated the protein expression levels of Nrf2 and inhibited the protein expression level of Keap1.These results indicated that LSA activated Nrf2 signaling pathways of intestinal mucosa.