Characterization And Gene Mapping Of Das Mutant In Maize (Zea Mays L.)

Plant height is an important trait in agricultural production and crucial for crop breeding.Properly reduced plant height could confer crops stronger lodging resistance,reduce losses by natural disasters and increase enhance yield.Therefore,it is important to elucidate the genetic mechanism underlying plant height for increasing of crop yield.In this study,we obtained a maize mutant,dwarf and stiff?das?,from natural variation.The mutation causes the reduced plant height to less than one-fourth of the wild-type.The internode number and the length of the mutant are reduced,the leaves are dark green and midribs are chlorotic.The ear of das mutant could barely set normal seeds,while the tassel could produce viable pollens.F₂ population of das and inbred line 178 for and InDel markers were developed from genome re-sequencing data of das mutant and inbred line 178 were used to identify the causative gene with map-based cloning approach.?1?We used the maize inbred B73 genomic data as reference sequence,and compared the re-sequencing results between the mutant and wild type to develop InDel markers.Ultimately,about102 polymorphic markers on 10 chromosomes and were validated and the candidate gene was localized on chromosome5 by primary mapping.?2?38 polymorphic markers were developed for fine mapping,and candidate gene was narrowed down to a 700 kb.?3?Hormone treatment experiments showed that the mutants were sensitive to the Gibberellin.The phenotype of the mutant could be partially restored by application of Gibberellin.