The Analysis On Host Localization And Odorant Binding Proteins Of Nasonia Vitripennis

Nasonia vitripennis(Hymenoptera:Pteromalidae)is a specific ectoparasitoid that attacks the pupae of fly species,including blow flies,flesh flies,and house flies.It is considered to be a biological method for flies control with great development prospects.The researches study the influence of antennae of parasitoid on host localization and host selection and try to find the area in antennae that works.The article uses molecular biology techniques to search the distribution of odorant binding proteins(OBPs)in antennae.The study also investigates the function roles of odorant binding proteins in host selection of the parasitoids.1.Influence of antennae on host localization of Nasonia vitripennisFirstly,behavioral approach is used to study the preference of Nasonia vitripennis to three kinds of pupae:flesh fly pupae,housefly pupae and fruit fly pupae.The results show the significant difference:the rate of choosing flesh fly pupae is highest with 68%and housefly pupae are second with 52%,while the rate of choosing fruit fly pupae is lowest with 12%.But the times to find those kinds of pupae have no difference.That means the parasitoids prefer flesh fly pupae than housefly pupae,and dislike the fruit fly pupae as it is not in the host range.Experiments of cutting the antennae of Nasonia vitripennis are conducted to find the functions of antennae in host localization.The results show the rate of finding the pupae is reduced after cutting the whole antennae,while the rate is rising after cutting part of the antennae.There are significant difference in rate and time of finding the host pupae between the experimental and control groups.2.A preliminary study of odorant binding proteins(OBPs)in antennae of Nasonia vitripennisThe real time quantitative PCR technique is used to define those odorant binding proteins(OBPs)that have high expression in the antennae of Nasonia vitripennis.Then,12 genes of OBPs are chose to undertake the RNA interference experiment.The results show only two genes' interference efficiency is higher than 60%,and the others are lower than 50%.And observing the morphological feature of antennae of adult parasitoids after RNAi,there is no significant difference.The results infer that the efficiency of RNAi around 60%has no impact on these OBPs genes.