Response Of Tomato TPR Gene To Stress Stress And Interaction Of SlTPR4 (SGT1) Protein

Tomato(Solanum lycopersicum)is a kind of popular vegetable which has important application value and medicinal value.The various vitamins and trace elements are riched in tomato,and it can prevent vascular aging and reduce the incidence of cancer by eating tomato with long-term.In the course of evolution,tomatoes have a strong ability to resist abiot ic and biotic stress.However,when the inappropriate environmental conditions exceed the tolerance limit of plants,the development and growth of plant will be effected in long time.it has cause the wilting,necrosis,decay,etiolation and dwarf of plants under abiotic and biotic stress.TPR gene family is a type of gene containing TPR conserved motifs.It is widely involved in cell cycle regulation,gene expression,protein transport,mitosis,protein folding,steroid receptor function,RNA splicing,transcription inhibition,protein degradation,stress and other biological processes.In Arabidopsis,TPR is involved in high temperature stress response and development.In wheat,the TPR gene plays a significant role in cold and salt stress response.However,the function and interaction mechanism of tomato TPR genes is not clear.The main research results are as follows:(1)26 TPR genes family members were identified by bioinformatics methods in this study,and subcellular localization,phylogenetic evolution,conserved motif,tissue expression and GO analysis of tomato TPR genes were performed.Subcellular localization analysis showed that TPR proteins were mainly distributed in the cytoplasm,a few of which were located in the epicyte and periplasm.Conserved motif analysis indicated that TPR genes of tomato had the same conserved motif.Tissue expression analysis showed that expression of TPR genes in tomato was different in diverse developmental stages and tissues,indicating that the function of TPR genes were diverse.In addition,TPR genes can respond to a variety of biological and abiotic stresses.But specific response mechanism of TPR genes need further study.GO analysis indicated that TPR protein of tomato is related to plant signal transmission and protein folding.(2)q-PCR technology was used to detect the expression levels of TPR gene family members under biological stress(Botrytis cinerea disease)and abiotic stress(ABA,drought,salt and trauma).The results showed that tomato TPR genes responded to biological stress and abiotic stress,among which SlTPR2 and SlTPR4 were significantly changed.(3)By VIGS technology,the silenced plants of SlTPR2 and SlTPR4 were obtained and respectively treated under biological stress and abiotic stress.The content of superoxide dismutase,peroxidase and catalase of silenced plants were determined.The results showed that the resistance of silenced plants of SlTPR2 and SlTPR4 was decreased.(4)In this study,interaction between SlTPR4 and atp A which codes ɑ-subunit of ATP synthase CF1 was found.In order to explore the interaction relationship between atp A and SlTPR4,the yeast two hybrid and pull down experiment were carried out.The result further proves that the interactions between atp A and SlTPR4.The research results of TPR genes will provides a theoretical basis for disease resistance molecular mechanism of TPR genes research.