Investigation And Genome-wide Association Analysis Of Soybean Corelated Traits In Seedling Stage Under Sulfur Stress

Soybean[Glycine max(L.)Merr.]is originated in China and has played an important role being grain and oil crop.As one of the most important sources of plant protein,soybean provides human and animals amino acids,especially lysine,leucine,and threonine which are necessary for life activity.The content of sulphur-containing amino acids limit soybean nutrient value.In recent years,it has been one of the research emphases to improve the quality of soybean and to increase the content of sulphur-containing amino acids in special.Studys have shown that sulfur not only increases yields,but also raises content of fat and protein.However,the soil in our country lacks effective sulfur for many reasons,such as the improvement of crop filds,creation of new variety,increase of multiple cropping index which leads to sulfur loses of soil.Besides,return amount is far more less than we take from soil due to improper way of fertilization.Farmers are conditioned to use a large amount of nitrogen fertilizer and phosphate fertilizer.They barely use organic fertilizer especially fertilizer containing sulfur.Above all,the phenomenon of soil sulfur deficiency is becoming more and more serious.Sulfur is one of the most important nutrient elements for soybean growth.It is of great significance to study the low sulfur stress in soybean.Many traits of crops are quantitative,such as yield,variety and stress resistance.The method of association analysis for the complex characters of plants has become more and more mature.With the development of genome sequencing technology and the decline of cost and the rapid development of bioinformatics,the application of association analysis in crop inheritance breeding is increasingly wide use.However,there are few studies on screening,identification and utilization of low sulfur tolerant genotypes.A lot of rich and good resistant genes need to be further excavated.In this study,a soybean natural population containing 219 materials from different sources is investigated using sulfur tolerant cultivation method of indoor hydroponics,looking into correlated traits during seedling stage,evaluation on different traitssystematically,screening out sulfur stress extreme materials according to different phenotypic character.Association analysis is conducted using 292053 SNP markers covering the whole genome of soybean,in order to identify significant SNPs affecting morphological traits of soybean seedlings and ones associated with low sulfur stress,in the end predicting some candidate genes by public database.Result shows,phenotypes such as plant height,root length,biomass,chlorophyll and so on have significant differences so that they can be used as reference markers for sulfur stress research.46 sulfur stress extreme materials were screened out according to these phenotypes.50 out of 1110 significant SNPs are repeatedly associated with in multiple environments and multiple traits,which distribute in 3,4,5,14,and chromosome 18.There are 13 candidate genes identified,including Glyma04g39370?Glyma04g39380?Glyma04g39390?Glyma05g04310Glyma05g04590?Glyma05g04600?Glyma05g31870?Glyma10g02540?Glyma12g34030?Glymal4g06981?Glymal4g07020?Glymal4g34856 and Glymal8g07220,most coding enzymes,and some coding transcription factors and transporters.