Identification And Analysis Of MADS-box Transcription Factors Related To Tuberization In Potato

Potato(Solanum tuberosum L.)is an important crop with high yield and adaptation to diverse environmental conditions.The tuber of potato is not only an economic organ but also a reproductive organ.Studying the mechanism of tuberlization provides a guide of improving the yield and quality.The photoperiod-respond CO-FT pathway regulates multiple developmental processes such as flowering,seasonal growth,and storage organ formation.The FT homologous gene St SP6 A mediates photoperiodic signals and regulates tuberlization.However,it is unclear that how St SP6 A further regulates the expansion of the tip of the stolon.Based on the conserved FT-AP1 regulatory module in the apical meristem,this study identified all of MADS-box genes in the whole potato genome and clarified its gene structure and expression patterns under different photoperiod conditions.Screening MADS-box transcription factors that may be involved in the regulation of photoperiod-induced storage organ formation by St SP6 A signaling can lay the foundation for investigation of signal transduction of St SP6 A.The major results are as follows:1)132 MADS-box transcription factors in potato were identified and analyzed for clustering,chromosomal location and gene structure by utilizing the plant TFDB database.The results showed that MADS-box transcription factors were clustered in two subfamilies: MIKC-type and M-type.M-type was further divided into three subfamilies: M?,M? and M?;MADS-box has similar genetic structure in the subfamily,and it is unevenly distributed on the 12 chromosomes of potato with the phenomenon of a clustered distribution.2)Through the analysis of the expression data of DM1-3 and RH which is published in the potato genome database spud DB,56 MADS-box transcription factors that expressed in stolons or tubers were screened.24 MADS-box transcription factors among them differentially expressed in four stages of tuberlization were chosen.And the 24 MADS-box transcription factors were further analyzed under different photoperiod conditions.We found that 10 MADS-box transcription factors differentially expressed under short day.10 MADS-boxes were further verified in The phytochrome F interference plant,and the results were consistent with those of photoperiod.3)The promoter cloning analysis of the 10 MADS-box genes we obtained showed that all of them contained the b ZIP family transcription factor core binding element.The cis-elements predicting results of the 10 MADS-box genes? promoter regions showed that there were differences among diverse MADS-box functional elements,but they all have their own light responsive elements.4)The TAC does activate the expression of five AP1 like genes and was confirmed by Agrobacterium-mediated transient expression of Nicotiana benthamiana;The subcellular localization results of DMT63313 and DMT68325 showed that they were localized in the nucleus.