The Effect Of Matrine Combined With Baicalin On Mice Pneumonia Induced By LPS

Objective:The aim of this study was to investigate the effect of matrine combined with baicalin on lipopolysaccharide(LPS)induced lung injury in mice,and provide data support for clinical application of matrine and baicalin.Method:42 mice were randomly divided into 7 groups,including:control group,LPS group,matrine group(30 mg/kg),baicalin group(200 mg/kg),combined high dose group(matrine 30 mg/kg+baicalin 200 mg/kg),combined middle dose group(matrine 15 mg/kg+baicalin 100 mg/kg)and combined low dose group(matrine 7.5 mg/kg+baicalin 50 mg/kg).A mouse model of acute lung injury was established by intraperitoneal injection of 20 mg/kg LPS.Various concentration of baicalin,matrine and matrine combined with baicalin were injected intraperitoneally 30 min after the administration of LPS.After 24 h of LPS challenge,serum was collected to examine the levels of interleukin(IL)-6 and tumor necrosis factor(TNF)-a by ELISA.The lung tissues were isolated for observing pathological changes by hematoxylin and eosin(H&E)staining and measuring the IL-6 and TNF-a mRNA levels by RT-PCR.MPO activity was determined by MPO activity kit.Results:Our data demonstrated that the intraperitoneal administration of LPS significantly damaged pulmonary function and induced inflammatory cells infiltration into the lungs.Compared with control group,the levels of IL-6 and TNF-a in both serum and lung tissues were increased(P<0.05,P<0.01).Compared with LPS group,pulmonary function in compound treatment groups was improved,and the levels of IL-6(P>0.05)and TNF-? in both serum(P<0.05)and lung tissues(P<0.01)were significantly decreased.The MPO activity in compound treatment groups were reduced(P<0.05,P<0.01).Conclusion:1)Matrine and baicalin have obvious anti-inflammation.2)There is no significant difference between 7.5 mg/kg matrine combined with 50 mg/kg baicalin and 30 mg/kg matrine alone group or 200 mg/kg baicalin alone group.3)Considering with the dosage and efficacy,the low dose of matrine combined with baicalin have more economic benefit,and need the minimum cost than other groups,so the low dose group is recommended in future clinical application.