Effects Of Physical Exercise On The Skeletal And Nerve System Of Developmental Mice Exposed To Fluoride

Accumulated studys from China and other other country truly elucidate skeletal and dental fluorosis and neurotoxicity of fluoride.additionally physical exercise have positive effects in bone system.And it could induce discrepancy of structures and functions in hippocampus and cerebral cortex.we expect to investigate the effect of physical exercise in growth period of mice with excessive fluoride.The present study was designed to explore the effects of physical exercise on skeletal and nerve system of fluoride intoxicated mice.Fourty healthy ICR mouse pup were randomly divided into four groups of control,physical exercise(PE),F,and F+PE from day 21.Mice in F and F+PE groups were received 100 mg/L naf in drinking water.The pup mice in F+PE group were exercised at the speed of 5m/min for 2 h/day.And animals maintained in groups of 10 per cage were fed standard chow and given drinking water for 7 weeks from postnatal day 21.The results show that 100mg/L naf significantly increased the ALP activity of mice,severely altered normal order of bony plate in femurs of mice.meanwhile,we examined PSD of synapsis in hippocampus and cerebral cortex with transmission electron microscope(TEM).This study present that PSD was reduced compared with control.And,furthermore fluoried changed mitochondrial ridge structure.Expressions of mrna in bone and nerve tissue also indicates naf increasing the risk of animal body.The levels of the ratio of RANKL to OPG(RANKL/OPG),Osterix and RUNX2-I in femur and rid of mice exposure to 100 mg/L naf have obviously differentiation.similarly fluorid impacted the mrna expressions of PSD-93 and PSD-95.The daily PE reversed the changes in ALP activity,promoted PSD amount and regained organization structure of naf.The findings in this study suggested physical exercise could weaken the impacts of fluoride in bone and nerve system through regulating the expressions of gene.