Differential Expression Analysis Of Transcriptome In Mouse Macrophages Infected With Edwardsiella Piscicida

Edwardsiella piscicida is an important pathogen in aquaculture.It has a wide range of infected hosts and can cause more than 20 kinds of fish infection-related diseases,causing huge economic losses to the aquaculture industry.In addition,it can cause disease in amphibians,reptiles and even mammals.Previous studies have found that fucose signally regulates the FusKR two-component system,regulates the transcription and expression of virulence factors such as the type III secretion system(T3SS),playing an important role in bacterial colonization of host cells and bacterial pathogenicity.However,the role of the key gene fucP in the infection process has not been clear.Therefore,in this study,mouse macrophages were infected by the wild-type strain E.piscicida EIB202 and the deletion strainΔfucP,and the differential expression of the transcriptome was performed by transcriptome sequencing and related bioinformatics analysis.To clarify the functional changes of immune-related signaling pathways and the specific role of gene fucP in the infection process.The results of transcriptome analysis showed that the total number of reads(Total Clean Reads)was 24,115,618 in the control group,24,111,407 in the EIB202 group,and 24,114,873 in the ΔfucP group,totally of 16085 genes were detected.Compared with the control group,the wild-type strain EIB202 screened 2610 differential expressed genes,of which 980 were up-regulated and 1630 were down-regulated.Compared with the control group,the deletion strain ΔfucP screened 2994 differential genes,of which 1106 were up-regulated and 1888 were down-regulated.Compared with the wild-type strain EIB202,the deletion strain ΔfucP had 66 differential expressed genes,47 up-regulated genes and 19 down-regulated genes.GO functional analysis results showed that differential expressed genes were annotated into three major categories.Among them,the number of differential expressed genes such as cell processes,biological regulation,metabolic processes,andstimulus responses in biological processes accounted for a large proportion.The cell group was classified into a large number of differential expressed genes in cell components,cells,organelles,and cell membranes.Among the molecular functional classes,there were many differential expressed genes in the catalytic activity and binding processes.Pathway analysis showed that the wild-type strain EIB202 group,2610 differential expressed genes were enriched in 44 pathways.Among them,four kinds of pathways were related to cellular processes,three kinds of pathways were related to environmental information processing,four kinds of pathways were related to genetic information,11 kinds of pathways were related to human diseases,12 kinds of pathways were related to metabolic processes,and 10 kinds of pathways were related to organic systems.Among them,there were many differential expressed genes in signal transduction system pathway,cancer pathway,and immune system pathway.In the ΔfucP group of the deletion strain,the enrichment results of2994 differential genes in the pathway were basically the same as those in the wild-type strain EIB202.In the wild-type strain EIB202 group and the deletion strain ΔfucP group,the number of up-regulated differential expressed genes were mainly belong to the NOD-like receptor signaling pathway and the cytokine receptor interaction pathway,and the number of down-regulated differential expressed genes were mainly belong to cancer pathway.Compared with wild-type strain,there was 66 differential expressed genes the deletion strainΔfucP which were enriched in 27 pathways.Among them,three kinds of pathways were related to cellular processes,two kinds of pathways were related to environmental information processing,two kinds of pathways were related to genetic information,11 kinds of pathways were related to human diseases,five kinds of pathways were related to metabolic processes,and four pathways were related to organic systems.At the same time,the number of differential expressed genes in the NOD-like receptor signaling pathway was the highest,and that in the TNF singnaling pathway,carbon metabolism,biosynthesis of amino acids,lysine metabolism,alanine,aspartic acid metabolism,the citrate cycle,and the terpenoid backbone biosynthesis enrichment pathway were up-regulated.