Irisin Promotes Mice White Adipose Cells Browning By Activating AMPK?1

[Purpose] Research showns that mammals store excess calories in the body as white tissue.Excessive energy intake is the main cause of obesity.Obesity is one of the most common metabolic diseases in the world.The incidence of type 2 diabetes and cardiovascu Lar diseases caused by obesity is also increasing year by year,so obesity It is a hidden danger that threatens human health.How to effectively and healthy limit excessive white fat in the body to treat obesity is also a hot topic of current research.In addition,excessive white adipose tissue in the pig industry also seriously affects the quality and economic benefits of pork.Studies have shown that white adipose tissue in animals has a benign tendency to brown adipose tissue conversion under certain conditions(sports,cold,etc.).Irisin is a small molecu Le protein secreted by skeletal muscle and plays an important role in the browning adipose tissue cells.However,its signaling pathway to promote browning remains to be further studied.This study aimed to investigate the signaling pathways that Irisin promotes browning of white adipose tissue.[Methods] In this study,3T3-L1 cells and mouse primary adipocytes were cu Ltured in vitro,differentiated with cocktail method.Then,AMPK?1-si RNA was transfected and 20nmol/L Irisin protein was added for treatment.Oil red O staining was used to identify the lipid droplet.RT-PCR,Western Blotting were used to detect brown adipose tissue marker gene uncoupling protein-1(UCP1),peroxidase-? coactivator 1?(PGC-1?),and the lipid droplets were detected by optical density analysis in white adipose cells.[Resu Lts](1)The resu Lts of q RT-PCR showed that the relative expression of AMPK?1 m RNA in white adipose cells was significantly lower than that in the control group after transfection with AMPK?1-si RNA.The q RT-PCR resu Lts showed that the relative expression of PGC-1? and UCP1 m RNA in the transfected group was significantly lower than that in the two adipocyte models.In the control group,Western Blotting showed that the relative expression levels of PGC-1? and UCP1 in the transfected group were significantly lower than those in the control group.(2)Oil red O staining resu Lts showed that lipid droplets were stained in both white adipose cell models;optical density analysis showed that there was no significant difference in OD values between the added and non-added groups in the white adipose cells after transfection of AMPK?1-si RNA.The resu Lts of q RT-PCR showed that the relative expression of PGC-1? and UCP1 m RNA in the transfected group was significantly lower than that in the non-transfected group.The resu Lts of Western Blotting showed that the two adipocytes were added with Irisin and The relative expression levels of PGC-1? and UCP1 proteins in transfected group were significantly lower than those in the non-transfected group.[Conclusion] AMPK?1 protein plays an important role in promoting the browning of white fat cells by Irisin,which provides a basis for further study of the signal pathway of Irisin to promote browning of white fat.