Study On Breaking Dormancy Factors And Changs Of Antioxidant Enzyme Activities Of Paeonia Rockii Seeds

Paeonia rockii is a national grade 3 protected plant of paeonia lactiflora.It has high value in medicine,ornamental and oil use.However,due to the dormancy of the seeds of Paeonia rockii,the seed propagation has such problems as long germination duration,uneven seedling emergence and low germination coefficient.Therefore,this experiment took the seeds of Paeonia rockii as materials to study the optimal method to break the dormancy of its hypocotyl and epicotyl,as well as the change of antioxidant enzyme activity during seed germination,so as to provide technical supportfor the rapid cultivation of high-quality seedlings of Paeonia rockii.The results show that:1.The rooting effects of different treatments were different.The rooting rate of seeds treated with concentrated sulfuric acid for 3min was 38.9%.When the light intensity was 3000Lx and the processing time was 16h,the rooting rate was 89.3%.When the temperature is 25?,the processing time for 24 h,the rooting rate is highest,at 90.9%.GA3 treatment can accelerate the rooting speed and improve rooting rate.Among them,300 mg·1-1GA3 treatment time of 24h was the most favorable for the rooting of peony puiple-spot seeds,and the rooting rate was 93.7%.2.Different treatments had different effects on seed germination of Paeonia rockii.The germination rate and germination potential were 93.4%and 82.9%at 30d.The germination rate and germination potential of 300 mg·1-1GA3 solution for 24h were 93.2%and 89.4%respectively.The seed treated with 100 mg·1-1 ethylene for 24 h had a germination rate of 89.8%and a germination potential of 89.1%.o3.On the basis of single factor test,L25(56)orthogonal test method was used to study different lamination time,different concentration of GA3 and different treatment time,different concentration of ethrel and different treatment time.Among them,the concentration of laminate 30d and GA3 was 300mg·1-1,the treatment time was 12h,the concentration of ethrel was 200mg·1-1,and the treatment time was 24h,germination rate is 95.2%.4.During the seed germination of Paeonia rockii.,the activity of antioxidant enzymes showed different dynamic patterns.During the seed germination of Paeonia rockii.,superoxide gasification enzyme(SOD)and peroxidase(POD)showed a decreasing trend,while catalase(CAT)showed a gradually increasing trend.