Effects Of Endophyte On Metabonomics And WRKY Geneexpression Of Rice Seedlings Under Salt Stress

Salt damage is a major abiotic stress factor affecting agricultural production,and rice is one of the most widely planted food crops in the world.When rice suffers from salt damage,it will reduce production a lot,but endophytic infection can greatly improve the resistance of rice and alleviate its damage.However,the molecular mechanism is still unclear.In this study,we utilized fungal endophyte EF0801 to infect rice under Na₂CO₃ stress.The experiment was divided into four treatment groups:control group?CK?,endophyte treatment?J?,salt treatment?N?,endophyte and salt treatment?NJ?.The coupling technique of gas chromatography-time-of-flight mass spectrometry was used to detect differences in metabolic processes,and RT-PCR was used to determine the relative expression of WRKY family transcription factors in rice seedlings at the same time.The effects of endophyte on rice seedlings under salt stress were studied from the aspects of metabonomics and gene expression,which provided a theoretical basis for elucidating the alleviation of endophyte on salt-stress rice.The main research results of this study are as follows:1.A total of 48 kinds of metabolites were detected in the control group and other three experimental group samples.It includes 8 glycolytic and TCA cycle intermediates,10 amino acids,12 carbohydrates and polyols,8 organic acids,4 fatty acids,and 4 other metabolites.2.The endophyte-infected rice seedlings,on the one hand,accumulated amino acids,carbohydrates,polyols,fatty acids and other organic matters;on the other hand,enhanced the process of glycolysis to produce more energy in order to improve the growth of rice.3.Under salt stress,the endophyte-infected rice seedlings changed small molecular osmotic regulatory substances,turned amino acids to carbohydrates and polyols,and reduced glycolysis and TCA cycle to resist salt damage.4.The endophyte-infected rice seedlings declined the relative expression of WRKY17,WRKY27,WRKY30,WRKY66 and WRKY87,and raised the relative expression of WRKY53.5.Under low salt stress,the endophyte-infected rice seedlings reduced the relative expression of WRKY17 and WRKY66,and raised the relative expression of WRKY90;under high salt stress,the endophyte-infected rice seedlings reduced the relative expression of WRKY30,WRKY53 and WRKY87,and raised the relative expression of WRKY66.