Effect Of Taurine On The Apoptosis Of INS-1 Cells ERS- Mediated Induced By High Fat And High Glucose

Ingestion foods with high glycolipid content and irregular often lead to insulin resistance and islet beta cell dysfunction,which will lead to type 2 diabetes.And the apoptosis of islet beta cells play an important role in the occurrence and development of diabetes.The study found that endoplasmic reticulum stress was the main reason for the apoptosis of pancreatic beta cells.Taurine is an important free amino acid and has many biological functions.Our preliminary test showed that taurine could reduce the blood sugar,the apoptosis rate of islet beta cells,the protein expression of apoptosis factor Caspase12 and CHOP,and improve the insulin sensitivity.In this experiment,we use high fat and high glucose culture islet beta cell line INS-1 to investigate the effect of taurine on the apoptosis of endoplasmic reticulum stress,providing a theoretical basis for the use of taurine to prevent and treat human and pet diabetes.INS-1 cells from pancreatic islet beta cell line were cultured,and INS-1 cell lines were randomly divided into 4 groups: the blank control group(O group 1640 medium),taurine group(T group 1640 medium + 30 mM taurine),high fat high sugar group(H group 1640 medium + 16.7 mM glucose + 1.0 mM palmitic acid),taurine high fat high sugar group(TH group 1640 medium + 30 mM taurine + 16.7 mM glucose + 1.0 mM palmitic acid).Cell proliferation activity was detected by MTS;the apoptosis rate and the change of the cycle of INS-1 cell lines was detected by flow cytometry;the ultrastructural changes of endoplasmic reticulum in INS-1 cells were observed by transmission electron microscopy;and the protein expression of endoplasmic reticulum stress markers BIP,IRE1,P-IRE1(ser-724)and apoptosis related factors Caspase12 and TRAF2 protein in INS-1 cell lines were detected by Westren-blot.The experimental results showed that the MTS assay showed that the concentration of 16.7 mmol/L glucose +1 mmol/L palmitic acid was the best for cell culture,and the protective effect of 30 mmol/L taurine on cells was the most significant.And the activity of the cells added with taurine was obvious.It is proved that taurine can protect cell viability.The cell activity of taurine high fat and high sugar group was significantly increase than that of high fat and high sugar group.The rates of cells apoptosis induced by high fat and high glucose was significantly decreased by cultured 48 h.Electron microscopy found that the endoplasmic reticulum of INS-1 cells treated with high fat and high glucose was expanded and showed vesicles,and the structure of endoplasmic reticulum could be reduced after the addition of taurine.The protein expression of ERS related factor BIP in INS-1 cells increased significantly when high fat and high glucose was stimulated to with 12 h,but the protein expression of BIP was reduced after the addition of taurine.The protein expression of IREI and phosphorylated IRE1 as ERS markers increased obviously when high fat and high sugar was cultured 24 h,and the protein expression of BIP was significantly reduced after the addition of taurine;the protein expression of apoptosis related factors Caspase12 and TRAF2 increased obviously with cultured 48 h in high fat ang sugar.What's more,after adding taurine,the protein expression of INS-1 cells could be significantly reduced.Therefore,taurine can inhibit apoptosis by reducing ERS of INS-1 cells treatment with high fat and high sugar and the protein expression of apoptosis factors.