Genetics Analysis Of Resistance To Powdery Mildew And Stripe Rust And Molecular Detection Of Stripe Rust Genes Of Wheat Varieties(Lines)

Wheat is one of the most important food crops,but due to the influence of wheat stripe rust and powdery mildew,the yield of wheat is greatly reduced.Study on disease resistant varieties is the most effective,economical,and environmentally controlled means of disease control.But at present,due to the high variability of powdery mildew and stripe rust,the simplification of resistant wheat varieties,and the emergence of new toxic races,the ability of resistant varieties will continue to be disappearing.Therefore,figuring out the genetic laws and resistant genes of wheat cultivars are essential prerequisite of distribution of resistant cultivars.Genetic analysis of resistance to powdery mildew at seeding stage and stripe rust at adult stage was researched.Besides,effective molecular markers of Yr5,Yr9(1B/1R),Yr10,Yr15,Yr17,Yr18 and Yr26 were used to detect the distribution of resistance genes in the Australia 121 cultivars.1.The crosses between four cultivars and Mingxian169 were separately tested with Bgt races E09?E15 and E18 in seeding to identify the genetic of stripe rust resistance genes.The resistance of zhongliang2000461 and zhongliang96289 to E09 was controlled by a dominant gene;for yanzhan4110,two dominant genes conferred resistance to E18;for zhongyou626,two dominant genes conferred resistance to E15.2.The crosses between four cultivars and Mingxian169 were separately tested with Chinese prevalent Pst race CYR32 in adult-plant stage to identify the genetic of stripe rust resistance genes.The results indicated that the resistance of ttianxuan45?zhoumai18?zhognliang99293 and migntian07112 was controlled by a dominant gene to CYR32;the resistance of lantian18 and weilong369 was controlled by a recessive gene to CYR32;for xiaoyan120 and xinong056,two dominant genes conferred resistance to CYR32;for yiyang106 and lognyuan935,two recessive genes conferred resistance to CYR32;the resistance of KM787 was controlled by two recessive gene;for tianxuan46,a dominant gene and a recessive gene conferred resistance to CYR32;the resistance of qinnong28 was controlled by a dominant gene and two recessive gene;the resistance of kechen225 was controlled by twodominant gene and one recessive gene.3.The potential stripe rust resistance gene(s)in these entries were postulated by the closely linked markers of all-stage genes Yr5?Yr9(1B/1R)?Yr10?Yr15?Yr17?Yr18 and Yr26.Molecular detection results suggested that Yr5 may exist in 12(9.92%)varieties,and 15(12.40%),8(6.61%),3(2.48%),6(4.96%),28(23.14%)and 18(14.88%)varieties may contain Yr10?Yr15?Yr17?Yr18 and Yr26.Among the Australian wheat materials tested for stripe rust resistance genes,19 of them detected two or more stripe rust resistance genes simultaneously.This study is conducive to the screening of resistant materials with different disease resistance characteristics,laying the foundation for further breeding of varieties resistant to stripe rust.