Transcriptome Characterization Based On Rna Sequencing In Different Varieties Jujube Lines Infetced By Alternaria Alternata

Ziziphus jujuba Mill.is an important dried fruit tree species in China.The development of the jujube industry has a very positive effect on farmers’ high economic income and the promotion of a good change in the ecological environment.Jujube fruit disease is mainly harmful to jujube fruit.The incidence range covers all jujube production bases in the country,causing serious impact on jujube fruit.The yield and quality are seriously damaged,causing serious damage to jujube areas in China.In order to understand the changes in the transcriptome level of jujube fruits induced by A.altrenata,it is possible to identify the type,number,and function of important genes related to fruit shrinkage resistance in jujube fruit.The response mechanism under infection by A.altrenata is of great significance.In this study,jujube fruit anti-cone fruit ’Feng mi guan’,senile fruit disease ’Qiyue Xian’ and its water treatment control materials,and A.altrenata were used as inducing conditions by using transcriptome sequencing technology.And qPCR and other techniques from the molecular level of the jujube fruit in response to A.altrenata induced response mechanism,the molecular means to study the regulation of plant key genes and jujube breeding a certain molecular theoretical basis.The main results obtained are as follows:1.According to the genomic transcriptome sequencing procedure and analytical method,the samples were obtained as 4.88×107,using the date anti-cone disease ’Honey can’,senile fruit disease ’Juyenxian’ and their clear water treatment control materials.4.93 × 107,4.74 × 107,and 4.80 × 107 Raw reads.Filtering low-quality data yields high-quality sequences of 4.87 × 107,4.91 × 107,4.71 × 107,and 4.78 × 107,respectively.Among them,4.12 × 107,4.28 × 107,4.06 × 107,and 3.86 × 107 were compared to the jujube genome,and 3.71 × 107,3.49 × 107,3.27 × 107,and 3.31 × 107 were uniquely matched.2.Through transcriptome sequencing and DESeq2 software for differential gene analysis,FDR<0.05 and ∣log2FC≧ ∣2 were used to screen significant differences in genes.The results showed that 39 genes were up-regulated in the honeypot R1 VS R2 differential gene,and the genes were down-regulated.8 expressions.The genes of ’July Fresh’ S1-VS-S2 were up-regulated by 1754 and genes down-regulated by 603.These differential genes may play a key regulatory role in plant infection by pathogenic bacteria.3.GO function enrichment analysis of significantly differentially expressed transcripts revealed that the ’Honey Can’ anti-diarrhea disease(R1vs R2)was significantly differently expressed in the transcriptome and was significantly enriched in nine transcripts,Significantly differentially expressed transcripts in the S1 vs S2 transcriptome were enriched in 31 terms.4.Analysis of metabolic pathways in A.tenuissima infective susceptible material ’July Fresh’ can be found through glutathione metabolism,fatty acid synthesis and metabolic pathways,flavonoid synthesis,cysteine and methionine metabolism,JA Signals,Brassinosteroids(BRs),Salicylic Acid Signals,Ethylene,and other key genes in the pathway are regulated to protect against infection by pathogenic bacteria.5.Significant differential genes in A.tenuissima infestation resistant material ’honey pot’ include expansin-A1,GMP synthase,aquaporin NIP3-1,transcription Factor MYB21,Purple acid phosphatase 17,RPM1,Glucose-6-phosphate/phosphate translocator 2,Transcription factor bHLH30,Amino acid permease,Protein NRT1/PTR family,CCCH zinc finger domain protein,Sulfate transporter,Zinc transporter and other resistance genes.The 13 real-time fluorescence quantitative analysis of genes related to anti-diarrhea disease at different inoculation times revealed that the 13 genes in high-resistant and susceptible plants did not express as the time of inoculation prolonged.In the same way,this difference may have led to differences in the disease resistance and susceptibility of dates.