Analysis And Evaluation On Specificity Nutrition Of Chinese Kale Germplasm Resources

Chinese kale(Brassica oleracea var.alboglabra Bailey)is one of the specialty vegetables in southern of China.As an important metabolic product,sulforaphane is the natural active ingredient with the anti-cancer activity in vegetables;Carotenoids are also an important antioxidants.In this study,the sulphoraphane,carotenoids and lutein contents of 47 Chinese kale germplasms were analyzed by high performance liquid chromatography(HPLC);meanwhile,the myrosinase(MY)gene expression,which reltated to sulforaphane synthesis,was detected by fluorescence quantitative PCR,The diversity of Chinese kale germplasm resources was evaluated on the physiological and molecular level and select Chinese kale varieties suitable for cultivation in the south of China.1 Sulphoraphane content in Chinese kale germplasms was different,BOK15-20 had the highest content in the flower stalks,was 77 times than BOK15-40The part with the highest sulforaphane content is the flower stalks among 47 Chinese kales germplasm,stems were next and the leaves is the lowest.There was significantly difference among germplasms as well,BOK15-20 had the highest sulforaphane contents(478.08 ?g/g,429.27 ?g/g,249.95 ?g/g)in the flower stalks,stems and leaves,respectively.The highest sulforaphane content(6.19 ?g/g)of the flower stalks is BOK15-40;BOK15-42 had the lowest sulforaphane content(6.64 and 0.02 ?g/g)in the stems and leaves.47 germplasms were classified into 5 groups by cluster analysis based on sulforaphane contents of the flower stalks,4 groups on the base of sulforaphane contents of the stems and leaves.2 Carotenoids content in Chinese kale germplasms was different,BOK15-38 had the highest content in the flower stalks,was 3 times than BOK15-3The part with the highest carotenoid content is the leaves among 47 Chinese kales germplasm,flower stalks was next and the stems is the lowest.Significantly difference existed among germplasms,BOK15-40 and BOK15-34 had the highest and lowest Carotenoids content(9.01 and 3.33 ?g/g)in the leaves;BOK15-38 and BOK15-3,BOK15-39 had the highest and lowest Carotenoids content(6.91 and 2.10 ?g/g)in the flower stalks;BOK15-12 and BOK15-16 had the highest and lowest Carotenoids content(3.94 and 0.24 ?g/g)in the stems.47 germplasms were all classified into 4 groups by cluster analysis on the base of Carotenoids contents of organs.3 lutein content in Chinese kale germplasms was different,BOK15-38 had the highest content in the flower stalks,was 5 times than BOK15-13The part with the highest lutein content is the leaves among 47 Chinese kales germplasm,flower stalks was next and the stems is the lowest.The lutein content was significantly different among different germplasms.BOK15-38 and BOK15-13 had the highest and lowest lutein content(95.21 and 17.82?g/g)in the flower stalk;BOK15-41 and BOK15-45 had the highest and lowest lutein content(29.21 and 7.93?g/g)in the stems;BOK15-45 and BOK15-16 had the highest and lowest lutein content(244.04 and 66.93 ?g/g)in the leaves,respectively.47 germplasms were classified into 5 groups by cluster analysis on the base of lutein contents of the stems,4 groups on the base of lutein contents of the flower stalks and leaves.4 The MY gene expressed differently in Chinese kale germplasms,there was positive correlated between MY gene and sulforaphane contentRelative expression level of MY gene of the flower stalks was highest and lowest in BOK15-20 and BOK15-39;In the leaves,BOK15-20 had the highest Relative expression level of MY gene as well,BOK15-15 was the lowest.Relative expression of MY genes were very significant positive correlation with sulforaphane content through relationship analysis,showed that MY genes involved in the synthesis of sulforaphane,providing the basis for further screening high sulforaphane resources by molecular biology methods.