A Study Of The Anti-Inflam Matory Activity Of And Influence On The NF-Kappa B Signaling Pathways By Arecoline

Inflammation is a complex response which can protect the living tissue fromthe external damage.The main clinical symptoms of inflammation including redness,swelling,heat and pain.Although inflammation is a kind of beneficial protection of the body in usual,the excessive inflammatory reaction will be harmful to the body.Therefore,it is meaningful to develop novel anti-inflammatory drugs and its mechanism of action.There are two kinds of anti-inflammatory agents at present which are steroidal anti-inflammatory drugs(SAIDs)and non-steroidal anti-inflammatory drugs(NSAIDs).SAIDs represented by Dexamethasone possess strong anti-inflammatory activity but serious side effects,such as immune suppression.On the other hand,more and more adverse reactions on gastrointestinal tract,liver,kidneys and nervous system have been reported.In other words,available anti-inflammatory agents could hardly meet the needs of clinical t herapy.It is an urgent demand to find novel anti-inflammatory drugs with less adverse reaction and strong specificity.Rich herbal medicine resources in C hina provide a huge database to screen activity agents and it has been selected a large number effect ive anti-inflammatory drugs in clinical.These drugs owe various structures and anti-inflammatory effects with different mechanisms which provide a theoretical guidance and scientific basis to find and develop new anti-inflammatory drugs from traditional Chinese medicine.Arecoline is a kind of oily liquid with colorless and odorless which is an effectivecomponentextracted from Areca catechu.It is usually used to treat glaucoma and expel tapeworm and it also has the treatment of displacement,postpartum uterine bleeding,uterine instauration and menorrhagia in clinical.There are lots of researches about the extraction process of arecoline and insect repellent effectat home and abroad,but less reported in other areas.Wehave found that arecoline has good anti-inflammatory effect in our anti-inflammatory drug screening experiment,but the anti-inflammatory potency and its specific mechanisms still uncertain.Therefore,anti-inflammatory activity and mechanism of action of arecoline is studied in this paper.In vitro experiment,The experimental Griess reagent method results show that the arecoline can obvious inhibit the production of NO which induced by LPS in RAW264.7 cells,and showed a dose dependent relationship.QPCR experiments showed that different concentrations of arecoline with different degree inhibition of IL-6,IL-1β,TNF-α and COX-2,i NOS on m RNA level which induced by LPS.Western Blot analysis results show that arecoline has significant inhibitory effect on COX-2 protein,cell nucleus factor related protein(P65)and the activation of IκB protein translocation.To sum up,the experiment proved that arecoline has better anti-inflammatory effect,and its action mechanism is via inhibit the activation of the NF-κB signaling pathways,then inhibit the production proinflammatory cytokines and inflammatory medium.