Effects Of ALDOB And HSP90B1 On Glucose Metabolism In DF-1 Cell

Muscle pH index of meat quality,which is determined by the initial content of glycogen in muscle and its lactate.Poultry are often affected by temperature leading to abnormal glucose metabolism.Previous studies have shown that ALDOB was involved in glycolysis,and also involved in the process of gluconeogenesis.When the protein structure was changes or decreased expression,it will cause a decrease in ATP content and the aldolase activity,thereby affecting the glycolysis or gluconeogenesis.HSP90B1,a member of the HSP90 family,is more study on involved in transferrin and plays an important role in lipid and protein metabolism.Some studies have shown that HSP90B1 is associated with changes in chicken muscle quality during heat stress.At present,there are few studies on the mechanism of heat stress changing pH value of chicken muscle.To explore of ALDOB and HSP90B1 on glucose metabolism in heat stress,we investigated the expression of ALDOB and HSP90B1 m RN A in different tissues under thermal stress,and overexpressed and knockdowned their mRNA expression at cellular levels,and detected the changes of glucose uptake and lactic acid production in our study.The results showed: The pH value of the breast and leg muscles of yellow feather chicken was significantly lower than that of non-heat stress.At 37?,ALDOB and HSP90B1 m RNA expression in the liver was higher than that in other tissues.After chronic heat stress treatment,ALDOB mRNA expression was increased significantly in all tissues except pituitary tissue,and HSP90B1 mRNA expression in each tissue was significantly increased.DF-1 cells were cultured at 37? as the control group.After stimulation with 41?,43? and 45? for 6h respectively,ALDOB and HSP90B1 m RNA expression were significant increased.The amount of glucose consumed and lactic acid produced were increased significantly.Overexpression of ALDOB could promote glucose consumption and increase lactic acid production at 37 ?.This phenomenon could be reversed while we interfering with the expression of ALDOB.After transfection of ALDOB overexpression vector or interference vector,there were no effect on the expression of HSP90B1.Under the condition of 43? heat treatment,overexpression of ALDOB will promote HSP90B1 mRNA expression,and the expression of ALDOB interference will down regulated HSP90B1 mRNA expression.The influence of glucose consumption and lactic acid production on the cell were similar to that in the control group.Overexpression of HSP90B1 could reduces glucose consumption and lactic acid production at 37?,this phenomenon could be reversed while we interfering with the expression of HSP90B1.But After transfection of HSP90B1 overexpression vector or interference vector,there was no effect on the expression of ALDOB.Under the condition of 43? for 6h,overexpression of HSP90B1 will reduces ALDOB mRNA expression,and HSP90B1 interference will promote ALDOB mRNA expression.The influences of glucose consumption and lactic acid production on the cell were similar to that in the control group.In summary,heat stress could decrease muscle pH and enhance the DF-1 cell glycolysis.The expressions of ALDOB and HSP90B1 mRNA were upregulated in multiple tissues or DF-1 cell under heat stress.HSP90B1 was involved in the process of glucose metabolism in DF-1 cell,which reduced the consumption of glucose and lactic acid production.