Effects Of Mixed Bacteria On Fermentation Quality And Digestibility Of Silage

In the recent years,silage has been valued by all countries in the world.In the sil age,the use of lactic acid bacteria can effectively reduce the p H of the feed,inhibit th e growth and reproduction of harmful microorganisms,so as to achieve the purpose of improving the quality of silage fermentation.However,due to the high cellulose cont ent of silage and the inability of lactic acid bacteria to degrade cellulose,the addition of lactic acid bacteria alone does not improve the digestibility of the feed.Currently,c ellulase preparations are mainly used at home and abroad to degrade cellulose and im prove feed digestibility.However,cellulase preparations are expensive and the prepar ation is complicated.In this paper,the main purpose of the enrichment culture of high-efficiency lactic acid bacteria and cellulose-decomposing bacteria for feeding and the effects of adding and mixing lactic acid bacteria and cellulose-decomposing bacteria separately on the additive effect of corn silage were studied.The main purpose of enrichment culture is to influence the mixed culture of fora ge lactic acid bacteria and cellulose decomposing bacteria with different addition amo unt of gibberellic acid.According to the experimental design,different addition amou nts of gibberellic acid solution were added to two equal strains of lactic acid bacteria(Lactobacillus plantarum and Leuconostoc dextran subspecies)and two strains cellulo se-decomposing bacteria(all Stenotrophomonas maltophilia)were enriched and cultur ed,the growth curve was drawn and the highest viable count was calculated.The exper imental results show that the experimental group(with addition of gibberellic acid:0.0%,0.5%,1.0%,1.5%,2.0%),the experimental group 2(with 1.0%gibberellic acid)viable count reached 1.16×10~9 cfu/m L,there was significant difference(P<0.05)com pared to the control group(1.62×10~8 cfu/mL),indicating that gibberellic acid had a sig nificant role in promoting the growth and reproduction of forage bacteria.Lactic acid bacteria and cellulose-decomposing bacteria with feed corn as raw m aterials were added and mixed separately.The experimental results showed that the p H value was control group(5.02),lactic acid bacteria group(3.77),cellulolytic bacteri a group(3.92)and mixed bacteria group(3.56)There was a significant difference bet ween the mixed bacteria group and the control group(P<0.05).The content of the lact ic acid bacteria was 1.58 mg/m L in the control group,6.080 mg/m L in the lactic acid bacteria group,and 3.172 mg/mL in the cellulose decomposing bacteria group.Mixed bacteria group(6.881 mg/m L),the mixed bacteria group had significant differences co mpared to other experimental groups(P<0.05);the content of neutral detergent fibers(NDF)was measured in the control group(65.10%),lactic acid bacteria Group(58.00%),cellulose-decomposing bacteria group(57.30%)and mixed bacteria group(54.30%),the mixed bacteria group had significant differences(P<0.05)compared with othe r experimental groups;the content of acidic detersive fiber(ADF)was the control Gro up(44.30%),lactic acid bacteria group(41.00%),cellulose decomposing bacteria gro up(39.50%)and mixed bacteria group(35.70%),the mixed bacteria group had signifi cant differences(P<0.05)compared with other experimental groups;The(ADL)cont ent is the control group(21.70%),The acid bacteria group(19.30%),cellulolytic bacte ria group(19.70%)and mixed bacteria group(19.10%)cellulose group had significant differences compared with other experimental groups(P<0.05);In vitro digestion experiments,dry matter(DM)digestibility was higher than the control group average of 11.02%in each experimental group,in which the cellulose g roup was significantly different(P<0.05)than the other experimental groups,and the neutral detergent fiber(NDF)digestibility was average in each experimental group co mpared to the control group.Above 17.48%,the mixed bacteria group was significant ly different from other experimental groups(P<0.05).The mixed addition of lactic acid bacteria and cellulose decomposing bacteria ha s a good promoting effect on the fermentation quality and digestibility of silage.This r esult will have certain reference value for the development of microbial feed additives and high-quality silage production and provide high-quality feed bacteria resources.