| Oil camellia(Camellia oleifera Abel.)is an edible oil tree species native to South China.Anthracnose is its main disease.Disease-resistant variety is the essential condition for development of oil camellia industry.However the underlying molecular mechanisms of disease resistance of the species remain unclear to date.MicroRNA(miRNA)is a kind of endogenous non-coding single-stranded RNAs with regulatory function,which is widely present in eukaryotes and has a length of about 20-24 nucleotides.It plays important regulatory roles in plant growth and development and stress response.In the present study,226 conserved miRNAs and 54 new candidate miRNAs belonging to 93 families in oil camellia were found by using high-throughput sequencing techniques combined with bioinformatic approaches,through analyses of expression profiles of miRNAs of oil camellia.The target genes of differentially expressed miRNAs in oil camellia were predicted,and functionally annotated using TargetFinder.Some miRNAs might be involved in protein synthesis,metabolism,response to stress and other processes in growth and development of oil camellia,including eight disease-resistant microRNAs.However,miRNAs and candidate target genes obtained still need to be validated using quantitative real-time PCR and stem-loop qRT-PCR,respectively in the future.The above results will enrich the information of oil camellia miRNA,provide the important basis for revealing the mechanism of disease resistance of the species.In addition,to supplement the molecular study,the contents in fruit and leaves of oil camellia cultivars with different disease resistance,such as soluble protein,soluble sugar,polyphenol,anthocyanin and phenylalanine ammonia-lyase,were analysed at the physiological and biochemical level.Results also indicated the differences of the five contents between oil camellia cultivars with different disease resistances,showing accordance with the previous related research. |
PDF Full Text Request