| Cotton is one of the major crops producing natural fibers and plays an important role in our national economy.However,the area of arable land continues to decrease in China,which has seriously affected the development of China’s cotton industry.According to statistics,the area of saline soil in China is 34.87 million hectares,about half area can be exploited.Therefore,exploring salt-tolerant genes in cotton,breeding high-quality,salt-tolerant cotton new materials through molecular breeding,and making full use of vast saline land to increase cotton yield have important implications for the development of China’s national economy and the cotton industry.In this study,use NaCl and Forskolin treatment salt-tolerant and salt sensitivity cotton plants such as P30 A preserved in laboratories,excavated a series of salt-tolerant candidate genes using bioinformatics tools,and the function of these genes was verified using the VIGS.A set of gene mining approaches from material processing,big data acquisition,rapid gene mapping of candidate genes to functional verification.The main research results obtained are as follows:1.Seed germination experiments showed that Forskolin,as an activator,can induce increased of salt tolerance during cotton seed germination.It is speculated that Forskolin may increase some salt-tolerant genes expression and increase the resistance of cotton seeds to salinity by activating cAMP cyclase activity.2.There are not only commonly expressed genes but also special expressions between samples during different germination periods and under different treatments.Under the NaCl+Forskolin combination treatment conditions,more positively regulated genes appeared,indicating that under the combined action of salt and activator,more genes are involved in salt-tolerance-related pathways,thereby enhancing the salt tolerance of seeds and increasing the germination rate of seeds.3.Ninety salt-tolerant related candidate genes were obtained by analysis.The results showed that after ST01,ST02 and ST04 gene silencing,the plants exhibited more significant salt sensitivity,presumed that these genes participation in salt tolerance process,and also verified the reliability of gene expression regulatory networks for screening cotton salt-tolerant related genes. |
