| By clarifying the molecular mechanism of rice seedling growth,it can provide reference and basis for humans to study the mechanism of plant growth and development.Maintaining sugar levels is critical for seedling survival.Sugar as a signal significantly affects the growth of seedlings.Glucose deprivation inhibits the growth of root and hypocotyl.Reasonable concentration of sugar promotes seedling growth,but sugar concentration above the threshold inhibite the growth of seedling root,cotyledon and hypocotyl.The study of key gene in sugar signal pathway Sn RK1 a revealed that the RNAi mutant of Sn RK1 a exhibited slower germination and inhibition of roots and shoots growth,in which root growth was inhibited more significantly.Osb HLH111 was a nuclear protein,Osb HLH111 was constitutively expressed in rice and has a relatively higher expression level in roots;Osb HLH111 inhibited the growth of rice seedlings,The length of 111-1/2/3 roots and the plant height were significantly increased by contrasted with the wild type,the root length and plant height decreased significantly in OE-1/2/3,while the sucrose and glucose contents increased in 111-1/2/3.The yeast two-hybrid experiment showed Osb HLH111 binds to Os Sn RK1 a.The bHLH transcription factor is one of the largest transcription factor families in plants.It is only known that PIFs in Arabidopsis participates in the process of hypocotyl elongation regulated by glucose signal,but the mechanism is unknown.Rice Osb HLH111 inhibits the growth of rice seedlings and Sn RK1 a is a key component of sugar signal transduction.Therefore,it has great significance to elucidate that the interaction between Osb HLH111 and Os Sn RK1 a participates in sugar signal pathway and regulates the growth of seedlings,this study will complete the sugar signal pathway and the seedling growth mechanism.The results of this study provide new experimental evidence and theoretical support for the regulation of seedling growth by sugar signal. |
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