Effects Of MeSA And MeJA On Morphology And Physiological Characteristics Of Hosta Ensata

Hosta ensata is an important ornamental plant in the landscape of northeastern China,The strength of its resistance is an important factor affecting its ornamental level.Therefore,Hosta ensata was used as a material in this experiment.The exogenous Methylis salicylas and Methyl jasmonate were used to treat Hosta ensata by spray method to explore the effects of different concentrations of exogenous substances and different treatment time on the morphology,osmotic adjustment substances,enzyme activities,and secondary metabolite substances of Hosta ensata.The main results of the experiment are as follows:1.The Hosta ensata height,leaf width,and leaf length after treatment with different concentrations of MeSA and MeJA were different from those in the control group,but they did not reach significant levels.Respectively,indicating that MeSA and MeJA had no effect on the growth of Hosta ensata significant role in promoting or inhibiting,no effect on the ornamental of Hosta ensata.2.Different concentrations of MeSA and MeJA could promote and inhibit osmotic adjustment substance in leaves of Hosta ensata persicae,but in general,the inhibition was more obvious.High concentrations of MeJA induced a decrease of soluble protein,soluble sugar and proline content in a relatively short period of time,and the inhibition of soluble sugar content was more obvious than that of MeSA.However,the content of soluble protein,proline inhibition is not obvious without MeSA.Thus,MeJA is superior to MeSA in inhibiting soluble sugar content,while its inhibitory effect on soluble protein and proline content is not as significant as MeSA.3.Different concentrations of MeSA and MeJA could increase the activity of enzymes in Hosta ensata leaves,which indicated that MeSA and MeJA had a certain role in promoting the activities of these enzymes,but the effects of the two exogenous substances were different.MeSA and MeJA were able to induce PAL to reach higher enzymatic activity at lower concentrations,and the time to peak was also earlier than that of MeJA,except that the persistence period was slightly shorter than MeJA 1mmol · L-1MeSA and MeJA had the greatest effect on PPO and POD activity,but MeSA and MeJA induced higher PPO and POD enzyme activities in a shorter time,but the effect of MeSA on PPO activity-induced persistence compared with MeJA,there is no difference in the persistence period induced by POD activity 1 mmol · L-1 MeSA and MeJA could induce SOD to reach the peak of enzyme activity on the 3rd day,but MeJA induced a higher enzyme activity change of SOD at the same time compared with MeSA,and the persistence period was also longer.Although MeSA and MeJA require higher concentration and longer time to cause CAT activity to reach the peak of enzyme activity,the peak value of CAT activity is higher,only the retention time is relatively shorter.Thus,the effect of MeSA on the activity of PAL,PPO,POD and CAT was better than MeJA,but the effect of MeJA on the induction of SOD was better.4.Different concentrations of MeSA and MeJA could cause the increase the content of three secondary metabolites in Hosta ensata leaves,which indicated that MeSA and MeJA could promote tannin,flavonoids and phenolic acids.In this experiment,the promoting effect of this effect is about 15 days,but the effect of the two exogenous substances are different.Compared with MeSA,MeJA caused a higher content of tannin and flavonoids at a relatively lower concentration,only the tannin content peaked relatively longer.However,MeJA effect is more obvious,the lower the concentration in a short time can cause the phenolic acid content to reach a higher peak.Therefore,it can be found that MeJA is superior to MeSA on the promotion of tannins,flavonoids and phenolic acids in the secondary metabolites in Hosta ensata.