Function Of Glutaredoxin GRXS16 In Brassinosteroid-induced Degradation Of Chlorothalonil In Solanum Lycopersicum

The use of pesticides can help crops to resist pests,weeds and other biological stress,and ensure the crop yield.While excessive and unreasonable use of pesticides leads to a large number of pesticides residues in soil environment,atmospheric environment and crops,which brought a series of hazards to agricultural production,environmental security and food security.Therefore,it is important to identify the genes involved in the degradation of pesticides in plants,which is of great significance to effectively promote the degradation of pesticides and reduce pesticide residues.In this paper,we investigated the role of CGFS type glutaredoxin(GRX)in brassinolide(BRs)induced degradation of pesticides in tomato(Solanum lycopersicum L.cv.Zheza 809)and wild-type Condine Red,using the chlorothalonil(CHT)as the target compound widely used in facility production.In addition,the GRXS16 overexpressed tomato plant was obtained by the combination of transgenic technology.The effect of overexpressing GRXS16 on the degradation of chlorothalonil was further studied,and the important role of GRXS16 in the degradation of chlorothalonil was further proved.The main results are as follows:1.The function of encoding NADPH oxidase gene(RBOH1)on BRs-mediated degradation of CHT in tomato was studied by virus-induced gene silencing.The results showed that CHT or BRs could not induce the expression of detoxification-related genes after silencing RBOH1,and the glutathione content and glutathione S-transferase activity were inhibited and CHT residues increased after silencing RBOH1.CHT and BRs could up-regulate the expression of CGFS-type GRX,which was not detected in RBOH1-silenced plants.2.The effect of CGFS type GRX on CHT residues was studied.The results showed that at least two detoxification genes,glutathione content and glutathione S-transferase activity were up-regulated in GRXS14,GRXS15 and GRXS17 gene-silenced plants.Expression of detoxification-related genes,glutathione content and glutathione S-transferase activity in GRXS16 gene-silenced plants were inhibited.Compared with the control plants,the CHT residues of GRXS16 gene-silenced plants increased and the CHT residues of GRXS14,GRXS15 and GRXS17 gene-silenced plants kept the same level or decreased.3.The effect of GRXS16 on BRs-induced degradation of CHT was studied.After silencing GRXS16,the induction effect of CHT or BRs on pesticide detoxification-related gene,glutathione content and ratio,and glutathione S-transferase activity disappeared,and whether be treated with BRs did not affect the residue of CHT compared with the control plants.4.The GRXS16 overexpressed plants were obtained by transgenic technology.The overexpression of GRXS16 had no significant effect on plant growth.After CHT treatment,the expression of pesticide detoxification-related gene was up-regulated,as well as the total amount of glutathione and the activity of glutathione S-transferase,and CHT residue was decreased.