Biological Functional Analysis Of Ribosomal Protein L21(Rpl21) In Magnaporthe Oryzae

Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases in cultivated rice that affects rice production and leads to enormous economic losses.Magnaporthe oryzae was considered as a model filamentous fungi.The process of growth,the incidence characteristics and the epidemiological characteristics of this pathogenic fungi are well known.Through these characteristics,people can develop new prevention and control strategies on rice blast at the molecular level.In the previous study,Mitochondrial ATP-dependent Lon Protease(MAP1)may regulate the pathogenicity of rice disease by regulating interprotein.Therefore,it is of great significance to reveal the pathogenic process of Magnaporthe oryzae by explore the function of MAP1 interprotein.Rpl21(Ribosomal Protein L21)is one of the proteins that are interacted with MAP1.For further analysis,a RPL21 gene deletion mutant ?rpl21 was generated by agrobacterium-mediated transformation(ATMT)method.In order to locate the genes,we successfully obtained the subcellular localization mutant and found that the gene was located on the cell membrane or mitochondrion.Through the wild type and mutant phenotypes were analyzed,and found that the mutant is more sensitive of SDS,conidiophores terrier,spore quantity increased,spore germination earlier,appressorium formation rate increased,pathogenicity of rice enhanced and the rice leaf sheath hypha extending more widely.We have obtained the target protein through prokaryotic expression and protein purification.The protein was injected into tobacco leaves and the tobacco leaves were allergic to anaphylactic necrosis.According to the above studies,RPL21 gene plays an important role in regulating the growth of mycelium and the formation of pathogenicity of the spores.