Genetic Relationship And Specific Marker Of Hybrid Brassica Napus Rape

The planting area of rapeseed(Brssica napus)in China is the largest among the oil-bearing crop.Nearly 70 years,the breeders of rapeseed have done a lot of researchs on rapeseed to improve the cultivars' quality and to breed a lot of new varieties with good qualities.However,the phenomena that some disordered rape species appears,what makes it difficult to breed high quality rapeseed.In this study,in order to identify rapeseed with a quick and simple and accurate method,a set of 50 rapeseeds with high quality from HuBei planting base were planted in GuangXi Nanning,base on that,3 rapeseed which survive good in Nannning were chose to identify germplasm resource with the molecular method of RAPD-SCAR.This study supply material basis of molecular marker indoor in purity indentification to the 3 rapeseeds survived well in Nanning,meanwhile,creating conditions for fammers'right to protect the quality of rapeseeds.The main result of the study were as follow:1.Successfully introduce 50 rapeseeds from planting base of HuBei,all of the 50 rapeseeds vernalized successfully after cultivating in the field.After further observation among the 50 rapeseeds,37 varierties are fertile,13 varierties are unfertile.2.63 different primers was chosen from 100 primers,the result of PCR have specific sequences by gel electropherosis,what can be used to distinguish the 5 rapeseeds.The size range of PCR result is 100bp-160bp,the range of band number of PCR amplification is 2-19.Co 63 amplification of RAPD primers is 451,all the 451 is polymorphic band,what can indicate the rapeseeds are polymorphic.3.50 rapeseed which cultivated in Nannning were chose to identify germplasm resource with the molecular method of RAPD,the culstering result analysed by UPGMA show:The total of 50 samples were divided into three categories.There is 20 rapeseeds in the firs group,and 9 of them can be fertile.There is 25 rapeseeds in the second group,and 20 of them can be fertile.There is 5 rapeseeds in the third group,and 2 of them can be fertile.4.4 specific sequence of RAPD molecular maeker were chosen from high yield and high quality combination of 5-13009×5-1135 and T11×5-1135.retrieve the specific fragment and clone them,obtain the sequnence number of the specific sequence afer sequencing.Design coressponding primer according to the sequnence number,in this way,RAPD marker can be sucessfully transformed to SCAR marker.5.Test the specificity of 3 rapeseed and 2 hybrid combo with the SCAR marker,the result show that the sequence can be amplified,what is same to the result predicted before.So,the new SCAR marker can be used to analyze the germplasm resource of productional seed,which is more simple and rapid.