Effects Of Co-inoculation With Trichoderma Asperellum And Ceriporia Lacerata On The Growth Of Soybean Seedlings

To explore the effect of co-inoculation with endophytic fungi Ceriporia lacerata and Trichoderma asperellum on soybean growth.In this paper,two highly salt-tolerant endophytic fungi,isolated from Kosteletzkya pentacarpos and soybeans were used in this laboratory to carry out pot experiment,and the best ratio of co-inoculation to promote soybean growth was first selected.The effect of co-inoculation and single inoculation on soybean seedlings under different salt concentrations were studied.We studied the effects of co-inoculation on enzyme activity,available phosphorus content in soybean rhizosphere soil,total nitrogen and total phosphorus content in soybean leaves and roots.We used fluorescence quantitative PCR to analyze the N/P nutrient utilization-related genes in dual inoculated soybean.The following main results are obtained:1.The biomass and physiological index of soybean after inoculation with fungi increased to varying degrees under different salt concentration conditions,and the co-inoculation treatment was better than the single inoculation,and there was a significant difference compared to non-inoculated soybean.When the salt concentration is 200 m M,the plant height of the co-inoculation soybean increased 27.22% compared to the non-inoculated control group,the fresh weight increased by 43.82%,the chlorophyll content increased by 22.92%,and the relative water content increased by 30.18%,MDA content of leaf and root decreased by 30.86% and 29.46% respectively.The activity of POD enzymes in the soybean leaves inoculated with Ceriporia lacerata,Trichoderma asperellum,and co-inoculation increased by 25.49%,30.56% and 47.02%,respectively,compared with that of non-inoculated soybean,and the difference was not significant between the two single inoculation,the activity of CAT increased by 22.11%,6.41% and 46.72%,respectively.2.The effects of enzyme activities and nutrient elements in soybean seedlings rhizosphere soil with co-inoculation were studied.After inoculation with fungi,the urease,protease,invertase and available phosphorus content in soybean soil were increased compared to the non-inoculated seedlings.The activity of urease in the rhizospheric soil with single inoculated Ceriporia lacerata,Trichoderma asperellum,and co-inoculation increased by 53.85%,9.16%,and 70.10%,respectively,the activity of amylase increased by 20.61%?16.58% and 52.74%,respectively,and the activity of protease increased by 92.96%?67.96% and 128.89%,respectively.The results showed that the effect of co-inoculation on soil enzyme activity were more significant.Compared with the non-inoculated,total nitrogen and total phosphorus in leaves and roots of co-inoculation soybeans had the most significant effect.Total phosphorus content in soybean leaves and roots increased by 77.01% and 64.61%,respectively,and total nitrogen content increased by 42.91%.,59.29%.3.To study the colonization of two fungi in soybean roots,we obtained the fungal transformation marked with the green fluorescent protein.By fluorescence observation,both fungi can colonize the root of the soybean.4.In order to further verify the effect of co-inoculation on the uptake and utilization nitrogen and phosphorus in soybean,fluorescence quantitative PCR was used to analyze the expression of some genes related to the utilization efficiency of nitrogen and phosphorus in soybean leaves and roots under low p/low N treatment.The single and co-inoculations had either up-regulated or down-regulated expression or no effect on soybean compared with the non-inoculated control.The situation is different between different genetic inoculation treatments.Compared with single inoculation,co-inoculation increased the expression of some genes more significantly,which is more beneficial to the transportation and utilization of nutrients by plants.