Study On Metabolic Recombination And Transcriptome Analysis Of V.uliginosum L. Under Low Temperature And Dark Culture

Bilberry,also known as blueberry,belongs to the genus Vaccinium L.of the genus Ectococcus from the family Eri-caceae.At present,the Food and Agriculture Organization of the United Nations ranks blueberries as one of the top five health foods.Blueberries have become the most promising fruit tree in the world.At present,the study of cold resistance of bilberry is a field with broad prospects but relatively weak research.Especially for the North,the cold winter weather is a very important thing.Bilberry cold-resistant methods generally use buried soil,greenhouses,etc.,which are time-consuming and costly.To realize the industrialization of blueberries,it is necessary to solve the problem of shortage of blueberry varieties.Compared with bilberry varieties,V.uliginosum L.are cold-tolerant wild blueberries distributed in the Changbai Mountains region of China.Therefore,in-depth study of the physiological mechanism and molecular mechanism of low-temperature dark blueberry cultivation is expected to provide a theoretical basis for the loss of cold-resistant varieties of modern bilberry cold-resistant breeds to provide a theoretical basis for the low temperature of the blueberry industry.(1)With the deepening of the cold stress,the physiological indicators of the two bilberry varieties showed the following degrees of no-use change,in which the relative electrical conductivity showed an upward trend;MDA showed a rising trend;and the tannin acid content also first increased.After the trend of decreasing,soluble sugar content showed an upward trend,soluble protein content increased first and then decreased,andSOD,POD and CAT activity showed a trend of increasing first and then decreasing.According to the comparison of the results of the physiological indicators of two kinds of cranberries,the “Muse” bilberry has a stronger cold resistance.(2)A transcriptomic analysis of the dark blue cultivar 'Muse' derived from cultivar cows revealed that 70,489(100%)were matched to the database,NR(42.72%),eggNOG(40.37%),GO(35.46%),Pfam(32.77%),KEGG(19.79%).There are 7239 differential genes,among which there are 3596 up-regulated genes and 3643 down-regulated genes.(3)Through analysis of metabonomics,the number of high-mass positive and negative ions was 10817 and 7353 respectively.In the treatment group,VT was compared with that of the control group VC,in which the number of differential positive ions was up-regulated by 32 and down-regulated by 106;negative ions were up-regulated.The number is 32,downgraded to 73.The metabolites were screened.Based on the similarity screening principle and analysis of variance,positive ions were used to screen out 139 differential metabolites and negative ions were screened for 105 differential metabolites.Among them,the biosynthesis of secondary metabolites dominates.(4)According to the joint data analysis of transcriptome and metabolome,there are 31 metabolic pathways that are co-enriched by differential genes and differential metabolites,in which transgenic plant hormone signal transduction and amino acid biosynthesis pathway are present.The differential genes were more 152,69.In this study,it is believed that the genes involved in plant hormone signal transduction and amino acid biosynthetic pathways are important genes for future research.