Effect Of Cumulus Cells On Oocytes About ROS And Antioxidase

In vitro cultures of oocytes and embryos are inevitably exposed to environments with higher concentrations of light and oxygen than in the body.This may lead to increased production of reactive oxygen species that are hindered by the cells and can destroy lipids,proteins,and nucleic acids thereby preventing Embryo development.Studies have shown that oxidative stress can cause immature cultures of isolated oocytes resulting in poor embryonic development.And the cumulus cells around the oocyte absorb and metabolize glucose,and the release of pyruvate and lactate may be achieved as energy release in the oocyte,so it is very important to protect the oocyte and embryo from oxidative stress.This dissertation intends to study the molecular level of oxidative stress in oocytes to investigate the effect of cumulus cells on antioxidant enzymes in oocytes.It is hoped that we can find ways to facilitate the in vitro culture of oocytes and their antioxidation.Changes in enzymes provide some results.In this experiment,three different culture methods of the experimental group(in vivo group,in vitro cultured group using artificial naked eggs and in vitro culture of cumulus oocyte complex group)were designed to pass through different periods(GVBD,MI,and MII stages,respectively).o)The oocytes were compared and the ROS content in mouse oocytes was determined by DCHFDA and their mRNA levels were expressed by the antioxidant enzymes SOD1,SOD2,SOD3,GPX1,GPX2,GPX3,and GPX4 to investigate their development during oocyte development.The content and changes in the.Experimental results show:1.The content of ROS in the oocytes of the DOs group after artificial treatment was significantly higher than that of the COCs group,and both groups were higher than the in vivo group.2.Compared with the in vivo group,there was a significant difference in the mRNA expression of antioxidant enzyme in oocytes except SOD1 and GPX4 in oocytes during GVBD(P<0.05);Compared with the in vivo group,mRNA expression of antioxidant enzymes in oocytes except SOD2 and GPX1 were statistically significantly different(P<0.05);mRNA expression of all genes in two in vitro groups Statistically significant differences(P<0.05).3.In the oocytes at the MI stage,mRNA expression of all antioxidant enzymes in the oocytes was significantly higher than that in the COCs group(P<0.05);DOs and in vivo In contrast,the gene expression of antioxidant enzymes in oocytes was statistically significantly different(P<0.05);whereas,mRNA expressions of GPX2 and GPX3 were statistically similar in COCs group compared with DOs group.There was a significant difference(P<0.05).4.In the oocytes during the MII period,the expression of mRNAs other than SOD1 was significantly different in the COCs group compared with the in vivo group;the DOs group had antioxidant activity in the oocytes compared with the in vivo group.There was a statistically significant difference in the gene expression of the enzyme(P<0.05).There was a statistically significant difference in the mRNA expression of all genes between the COCs group and the DOs group(P<0.05).5.However,compared with the three groups,the mRNA expression trends of GPX2 and GPX3 in the oocytes were not changed.This shows that the change trends of GPX2 and GPX3 are not significantly related to whether there are cumulus and environment,as for other genes,COCs group Compared with the in vivo group,mRNA expression of the antioxidant enzyme gene in the oocyte showed a similar tendency in statistics,and compared with the in vivo group,the DOs group had an anti-oxidase gene in the oocyte.The mRNA expression showed a statistically opposite trend.