Transcriptome Analysis And Related Functional Genes Of Safflower Seedlings In Response To Salt Stress

As a multifunctional crop,safflower has enormous economic benefits in field of medicinal use,food consumption,and ornamental.Safflower is widely cultivated all over the world;the land salinization is one of the most important soil problems we are facing in the world today.In order to promote the cultivation of safflower in salinized regions,in this paper,physiological and biochemical responses and transcriptome studies of salt tolerance of safflower seedlings were performed.In this study,salt stress was applied to the safflower seedlings with salt concentration varied from 75 mM to 150 mM,respectively.The water content,chlorophyll content,free protein content and antioxidant enzyme activity(SOD,POD,CAT)of safflower seedlings were measured.The experimental results show that the water content of safflower leaves is decreased and the free protein content is increased with the increase of salt concentration,which indicates that the osmotic pressure is adjusted to cope with the pressure of environmental stress.At the same time,the chlorophyll content has increased,combined the change of physiological and biochemical response with KEGG pathway result,increase the light capture capability to enhance the efficiency of photosynthesis.Finally,antioxidant enzyme activity is also positively correlated with salt stress.Under salt stress,many ROS exist in safflower seedlings.In order to slow down salt stress,the expression of antioxidant enzymes is increased and active oxygen is eliminated.The experiment results shown that the safflower seedlings have salt tolerance to a certain degree.Transcriptome sequencing,shearing,splicing,and functional annotation on the salt stressed safflower seedlings,calculating the gene expression of each sample.And then deferentially expressed genes between samples were screened by log|FC|>1&&FDR<0.05,the number of up-regulated gene is 25,and down-regulated gene is 79.Finally,deferentially expressed genes were analysis by GO enrichment,KEGG pathway enrichment;we obtained the metabolic pathways related to safflower seedlings under salt stress.What's more,we also analyze the photosynthesis antenna protein and biohormone transduction pathway in detail,and find some key genes related to these pathway.