Study On The Morphology Of Digestive System With Different Processed Products Of Terminalia Chebula Retz In Mice

The digestive system is of great significance for the growth of animal.it is not only the major sites for digestion and absorption but also playing an important role in drug metabolism.Following ingestion of Terminalia chebula Retz(TC),the digestive system could be exposed to a high concentration of TC,also the processed products of Rubia cordifolia L.per Terminalia chebula(RTC)and Euphorbia fischeriana Steud per Terminalia chebula(ETC)have better efficacy than TC.Up to now,people have paid attention to the studies on the protective effect of digestive system of TC,and researches on these branches have made certain progress.However,limited information is available concerning the influence of TC and its processed products on the morphology and function of digestive by given TC.In this study,one hundred and eighty two twenty-one-day-old SPF Kunming mice were randomly divided into high TC,RTC,ETC groups(4.0 g·kg-1),low TC,RTC,ETC groups(1.0 g·kg-1)and control group(saline,0.1 g/10g)with twenty-six replicates per group for 28 days.The aims of the present study were to investigate the effects of TC and its processed products on the dynamic changes of the morphological structure,intestinal mucin and endocrine cells in mice' digestive system by light and electron microscopes,Alcian Blue-periodic acid-Schiff staining,Grimelius' argentaffin staining and immunohistochemical staining.The effects of TC and its processed products on the digestive system's function and its mechanism in mice were discussed.Research results are as follows:When compared with the control group,the significant increases of mice's body weight in the RTC,ETC and high TC groups at 28 days of drug delivery were noted(p<0.01 or p<0.05).Under the light microscopes,not any histopathologic changes appeared in digestive system and the intestinal villi of small intestine were intact in the dose group.When compared with the control group,the villus height,crypt depth and villus/crypt ratio in the RTC,ETC and high TC groups of duodenum,jejunum and ileum were significantly increased(p<0.01 or p<0.05)at 28 days of drug delivery(except for the crypt depth in the low ETC group and high TE group).The results of electron microscopes showed the microvilli were arranged in order,mitochondrial cristae were clearly appearing and the electron density was uniform of the absorptive cells,as well as the nucleus of hepatocyte was well-preserved and glycogen granules were abundant of the liver in the high RTC,ETC and TC groups at 28 days.The results of AB-PAS staining showed that the type ? and ? of goblet cells were increased in the drug delivery.A significant increase in the number of goblet cells in the RTC and ETC groups were observed in all intestinal segments except duodenum(p<0.01 or p<0.05)during the experiment,also these groups' goblet cells were markedly increased in all intestinal segments when compared with the low TC group at 28 days.Argentaffin staining analysis indicated that compared with the control group,the argentaffin cells in experimental groups were significant increased in the gastric and pylorus during the experiment(p<0.01 or p<0.05)except for the TC groups at 7 days and the low TC group at 14 days of drug delivery.Furthermore,the argentaffin cells in the RTC and ETC groups were significantly increased in the intestines at 21 and 28 days of experiment(p<0.01 or p<0.05).When compared with the control group,the protein expression of 5-HT and SS of the duodenum,jejunum and ileum in the RTC and ETC groups were observed significantly decreased during the experiment(p<0.01 or p<0.05)(except for 5-HT in the ETC groups at 7 days and in the low ETC group at 14 days).The significant increases of GAS's protein expression in the RTC and ETC groups were observed in the pylorus and duodenum during the experiment(p<0.01 or p<0.05)(except for the low ETC group at 7 days).In conclusion:giving RTC,ETC and TC in the dosage of 1.0?4.0g·kg-1 promoted the growth and development of duodenum,jejunum and ileum.It caused the increased expression of goblet cells,argentaffin cells and GAS.It also lead to the decreases in the expression of 5-HT and SS.These results suggested that the morphology and function of mice's digestive system might be affected.