Application Of A Low-temperature-resistant Bacillus Purifing Agent

Aiming at the wintering characteristics of dalian,From the local water extract a low-temperature-resistant Bacillus that can improve water quality degradation of ammonia nitrogen,COD and sulfur By artificial sewage experiment test,found that the degradation of ammonia nitrogen,COD and sulfur.And has carried on the sea cucumber breeding experiment,research that the degradation of bacteria liquid added in the aquaculture water.Analyzed community of intestinal and aquaculture water through high-throughput sequencing.Optimized the medium formula and culture conditions of the low-temperature-resistant Bacillus firstly.The conclusion of Optimization showed that the medium components were as follows:olublestarch 10g/L,lactose 20g/L,(NH4)2SO4 10g/L,ureophil 5g/L,Yeast extract 5g/L,yeast cream 10 g/L,KH2PO4 0.25 g/L,K2HPO4 0.75 g/L,MnSO4 0.05 g/L,FeSO4 0.1 g/L.The conclusion of fermentation conditions were initial pH 7.5,inoculum size 3%,rotate speed 180r/min.In these conditions,the number of living bacterium can reach 2.37×108 CFU/mL,which was the 4 times of 5.87×107 CFU/mL that before fermentation.Through artificial sewage experiment found that the low-temperature-resistant Bacillus can degradet COD,sulfide and ammonia nitrogen in artificial sewage,ammonia nitrogen was more obvious compared with COD and sulfide,from what we can concluded that the low-temperature-resistant Bacillus can decompose COD,sulfide and ammonia nitrogen in sewage.Through the sea cucumber breeding experiment showed that the strains of bacillus in low temperature of ammonia nitrogen and chemical oxygen demand(COD)in aquaculture water COD degradation effect,this is the same as artificial sewage water purification effect is the result of the experiment.To not present a degradation of the role of sulfide,this are different from artificial sewage water purification effect experiment.Analyzed community of intestinal and aquaculture water through high-throughput sequencing at last.The Stichopus japonicus intestinal flora and aquaculture water were mainly classified into 8 phyla and 99 genera,and the total number of OTU was 229.At genus level,In HS1,the 5th day of Stichopus japonicus intestinal,Rubritalea(45%)and Colwellia(22%)were predominantly observed,followed by Halomonas(10%);In FJY1,the 5th day of aquaculture water,Sulfitobacter(55%)was predominantly observed,followed by norank_f_Rhodobacteraceae(14%);In HS2,the 10th day of Stichopus japonicus intestinal,Pseudoteredinibacter(15%)and SAR92_clade(16%)were predominantly observed,followed by Bizionia(10%)and Persicirhabdus(8%);In FJY2,the 10th day of aquaculture water,Glaciecola(30%)and Sulfitobacter(25%)were predominantly observed,followed by Colwellia(20%)and Marinomonas(10%).In the kelp community,In HS2,Proteobacteria and Bacteroidetes were dominant kelp members,followed by Verrucomicrobia;In HS1,FJY1 and FJY2,Proteobacteria was dominant kelp member,followed by Bacteroidetes and Verrucomicrobia.