| Soybean(Glycine max(L.)Merr.),originated from China,is an important cash crop,and contains a lot of beneficial ingredients.Common cutworm(Spodoptera litura)broke out in several East China provinces in recent years;it is one of the main pests in soybeans.Meanwhile,with long-term use of pesticides,common cutworm has developed resistance.Therefore,effective and environmental friendly method is urgent needed,selective breeding resistant varieties have become the major way of pest management.This study regarded wild soybean and soybean landrace as materials,to dig the new resistance materials and the new QTLs.It can be used for the further resistance evaluation system、resistance mechanism and resistant breeding.In this study,a representive sample composed of 203 wild accessions,and 370 landrance varieties were used for rearing common cutworm.Common cutworm was reared in the laboratory,the newly hatched larva of S.litura as tester,weighed larva weight of different feeding duration(at 6th,9th and 12th day).In order to compare the different feeding duration’s varation,we turn the larva weight into membership index value based on the standard accessions,it can be used for evaluate the insect resistance of the representive soybean in each eco-region of china.Acorrding to the result,use the two-stage associated analysis to do the GW AS of wild soybean and landrance population.Then QTL-allele matrix was established based on the QTL allele effect.The main results are as follows:1)The results of joint ANOVA of the 6th day’s membership index value in 2014 and 2015 showed that there existed significant difference among/between accessions,years and AccessionxYear interactions.The results of joint ANOVA of different feeding duration in 2015 showed that there existed significant difference among different feeding durations and accessions,while AccessionxFeeding-duration interactions did not show significant difference.It indicate that the resistance between different accessions were significant different,among different years、different feeding duration the resistance level is discrepant,and there existed obvious difference among AccessionxYear interactions,but no significant difference among Accession x Feeding-duration interactions.The accessions were grouped into highly resistant,resistant,medium resistant,susceptible and highly susceptible grades using a standard check grade system.A significant relationship was detected between eco-region and resistance grade,more resistant wild accessions existed in Middle and Lower Changjiang Valley Eco-region(Ⅲ)and Southwest-Central South Eco-region(IV),while more susceptible wild accessions existed in North Eco-region(I).Ten highly resistant wild soybean accessions N23369.T960097-1、Zhongshanling-4.F-013、J-024、N23342、N23349.TY100-1、N23312 and ZYD4349 were screened out and ten highly susceptible wild soybean accessions N23264、N23433、N23440、N23444、N23457、N23352、N23417、N23424、N23434 and N23185 were screened out;the ten highly resistant wild soybean accessions performed higher resistance than the highly resistant cultivated soybean PI227687 and Lamar2)The mapping results of wild soybean contain 28 QTLs,explain 68.68%of the phenotypic variation,undected minor QTLs about 26.90%,it distribute except for Gml、Gm5、Gm7、Gm9、Gm11、Gml2 and Gm20.There are five major QTL which R2 are bigger than 4%,respectively as wCCW-a-03-3、wCCW-a-10-1、wCCW-a-13-1、wCCW-a-16-2 and wCCW-a-17-1,it can explain the phenotypic variation by 7.96、4.22、11.07、6.83 and 7.86%、respectively.Compare the mapping result to the former soybean leaf-feeding insect’s resistant QTLs,we found that there are eleven QTLs located in or near the position of the QTLs or markers already reported.3)The spring and summer sowing soybean landrace’s joint ANOVA results of the 6th day’s membership index value,showed that there existed significant difference among/between varieties,testes and Varieties×Testes interactions,the 9th day’s reared result showed the similar result.The results of different test and feeding duration’s joint ANOVA showed that there existed significant difference among different varieties、different test and different feeding-duration;Feeding-duration x Test interation shows difference and Varieties x Test interation shows significant difference while Varieties x Feeding-duration interactions did not show significant difference;the block which set in test and feeding-duration shows significant difference;feeding-duration、test and varities interaction shows no difference.The varieties were grouped into highly resistant,resistant,medium resistant,susceptible and highly susceptible grades using a standard check grade system.A significant relationship was detected between eco-region and resistance grade,there were no highly resistant material in all six eco-region.Resistant varieties mostly derived from Huanghuaihai Eco-region(II),and highly susceptible varieties distributed except for eco-region VI.Ten resistant soybean landrace varieties N06202、N24297、N00710.2、N09737、N24158、N24480、N23576、N23578、N23582 and N09962 were screened out;ten highly susceptible soybean landrace varieties N05157.2、N02840、N10201、N23847、N23597、N04671.2、N06166、N05094、N23533 and N24190 were screened out.4)The mapping results of soybean landrace contain 52 QTLs,explain 21.61%of the phenotypic variation,undected minor QTLs about 8.50%.Most QTLs were detected on Gm3 and Gm10;there are six and seven QTLs,respectively.There are two high QTL which R2 are bigger than 1.5%,respectively as vCCW-a-01-2 and vCCW-a-20-2;it can explain the phenotypic variation by 1.96 and 1.95%.Compare the mapping result to the former soybean leaf-feeding insect’s resistant QTLs,we found that there are nineteen QTLs located in or near the position of the QTLs or markers already reported.5)QTL-allele matrix of the S.litura resistance in wild soybean and landrace population were established,it contains all the genetic information of 203 wild soybeans and 370 landrace varieties.There are a certain amount of positive(the larva weight is heavy)and negative(the larva weight is light)allele in insect-resistant materials and susceptible materials in two population.Insect-resistant material contains relatively more negative allele and susceptible material contains relatively more positive allele.The phenotypic effects of wild soybean material ranged from-0.41 to 0.48.The phenotypic effects of soybean landrace were ranged from-0.19 to 0.22.6)The result of this study showed that there are no same QTL between wild soybean and soybean landrace.It might because of the different population,and the different genetic background.Wild soybean has highly resistance accessions and unique QTLs.As for wild soybean will provide gene resource or allele varation for soybean landrace. |
