The Functional Analysis Of CaC3H1 In Pepper Response To Ralstonia Solanacearum Inoculation And Salt Stress

CCCH-type zinc finger is a key regulator of plant growth and development,as well as plant defense responses.A full lenghth cDNA gained from pepper(Capsicum annuum)cDNA library was termed as CaC3H1.Our data indicated that the CaC3H1 protein expressed through the whole pepper organization and localized to nucleus.Cell death response was found in pepper plants inoculated Agrobacterium-mediated transient expression 35 S:CaC3Hl accompanied by inducing of various pathogen-related genes.However,in the CaC3H1 pepper plants silenced by virus-induced gene silencing(VIGS)displayed decreased resistance to the highly virulent R.solanacearum strain FJC100301 with the down-expression of target genes,which is consistent to the results in transient expression.All these findings were further confirmed in the tobacco over-expressed CaC3Hl infected R.solanacearum.We also discovered CaC3H1 was was quickly and transiently induced by NaCl treatment.On the other hand,transgenic plants over-expressing CaC3H1 show reduced induction of salt stress-responsive genes and are more tolerant to salt stress.Taken together,these results indicate that CaC3H1 positive resistance to R.solanacearum and negative tolerance to salinity.