| Straw technology has become the major way of China,and He nan is the major agricultural province,Straw has also been popular,but there are a lot of problems,like the straw decomposition slowly,loose soil and affect plant seed germination,resulting in seedling yellow and weak,affecting the root development,and the straw can't corrupt in long-term will cause the population of pathogens,leading to the epidemic of soil borne disease,impact the agricultural production and rural incomes currently.straw degradation agents used on agricultural production rarely,Soil-borne diseases mainly used in chemical and agricultural control,But the effect is not ideal.Before there are also reported on the use of straw-degrading bacteria and the screening of oil-borne diseases biocontrol agents,but reports on the Bipolaris sorokiniana biocontrol agents is still less.this paper is aimed at the above problems,Screened and studied the high efficiency degradation bacterium and have the effect on the Bipolaris sorokiniana biocontrol,In order to speed up the decomposition and control of soil borne diseases using straw beneficial microorganisms,development of biological agents,and lay the foundation for popularization and Application.Specific experimental results are as follows:1,Screening and identification of the straw-degrading bacteriaIn 2015 were isolated from Shandong,Henan and other places to collect soil and litter samples,292 samples were preserved in our laboratory activation,use of the indoor specific medium screening,lignin cellulose enzyme activity determination,degradation rate determination and laboratory test method for the determination of initial screening of fungal strains with cellulose degradation ability 2 strains named X151,X125 and its degradation rate were 25% and 26.80%,have the ability of lignin degradation 1 strains were numbered XSBN-071-1,the straw degradation rate was 25%,The highest degradation rate of the combination of the two kinds of degrading bacteria is the combination of X125-XSBN-071-1,the degradation rate was 37.5%.The rate is 10.7% higher than the highest single straw degradation strains.The identification of cellulose degradation bacteria X125 is Diatrypella verruciformis degrading bacteria XSBN-071-1 identification for Cladosporium cladosporiodies.2,Screening and identification of the Bipolaris sorokiniana bio-control bacteriaCollected from various parts of the collected samples of 56 strains of the selected strains,finishing the activation of the laboratory to save the candidate strains of 206 strains,including fungi and bacteria.The specific medium room flat at the beginning of the screen,flat rescreening,pot experiment of Bipolaris sorokiniana pathogenic fungi of Bipolaris sorokiniana Helminthosporium and Fusarium graminearum has inhibitory effect of biocontrol screened out,which on false Fusarium graminearum has inhibitory effect of biocontrol fungi and bacteria,were screened elected 2 strains of fungi are XC-1,bacteria are XJ-17.XC-1,M-2 on Bipolaris sorokiniana creep spore fungus disease prevention effect better strains of fungi,bacteria are XJ-15.This experiment for the final effect is good strains extracted genomic DNA,then with universal fungal primers ITS1 and ITS4 for PCR amplification,biocontrol bacteria the 27 F and 1492 r were amplified by PCR,and observed the morphological characteristics,physiological and biochemical experiments of determination of the screened strains were identified.After identification,biocontrol fungi XC-1 wood for quasi Trichoderma koningiopsis,the strain M-2 for Trichoderma atroviride,biocontrol bacteria XJ-15,XJ-17 Bacillus.methylotrophicus with a kind of.We detected two kinds of Trichoderma and Bacillus methylotrophicus have strong antagonistic reaction,Not suitable for mixed use. |
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