Study On Sex Identification Of Actinidia Arguta By Physiological Biochemical And SRAP

Actinidia aruguta(Seib.Et Zucc.)Planch.Ex Miq is dioecious plant,which has brought a lot of inconvenience of artificial domestication cultivation and innovative use of germplasm resources because of cannot identify sex of Actinidia aruguta at seedling stage.In order to seek the effective method for sex identification of Actinidia aruguta,taking the toot and leaf of male and female Actinidia aruguta as experimental marerials,with determination the differences of physiological index,photosynthetic characters,isoenzyme and DNA between male and female of Actinidia aruguta.Thus,building the effective method for sex identification of Actinidia aruguta.The results showed that:(1)The BTB dyeing of male and female Actinidia aruguta has a significant difference,that is,the absorbance of female plant is significantly higher than male plant after reaction 4 h.The absorbance of female plant is significantly higher than male plant at 485nm by TCT dyeing;the anthocyanin content of male and female Actinidia aruguta has significant differences,which is the anthocyanin content of female strains is significantly about three times higher than male strains.The diurnal variation curves of female Actinidia aruguta’s net photosynthetic rate is bimodal type,and the male strain is triple peak type.The diurnal variation curves of female Actinidia aruguta’s transpiration rate is unimodal type while the male strain is bimodal one.The diurnal variation curves of female aruguta’s stomata conductance is bimodal type while the male strain is triple peak type.The curvilinear trend of diurnal variations of internal CO2 between female and male Actinidia arguta is basically consistent and both of them show three peaks,but the appearing time and values of the peaks are significantly different.(2)The peroxides isozyme of male and female Actinidia aruguta shows some differences on June 3(in the early period of florescence)that is the male plant presents four bands while the female shows three bands.Thereinto,the Rf of 0.12,0.17,and 0.38 are bands shared by male and female plant,and the Rf of 0.49 is the unique band for male plant in the early period of florescence.(3)On the molecular level,15 pairs of primers which can amplify polymorphic primer combinations strip stably are selected from the early 80 screen pairs of primers by the means of SRAP markers.Thereinto,the polymorphism ratio of female plants is 68.80%while male plants’ is 87.00%.The amplification results of four pairs of primers show that polymorphism ratio between male and female plants are significantly different(me2-em1,me6-em6,me7-em5,me7-em7).Dividing 19 tested plants clustering analysis into two groups,more than 70%of plants in every group are presented as the same sex.In conclusion,this study suggests that the BTB and TTC dyeing is an effective method to identify the male and female plant of Actinidia arguta;the anthocyanin content of female Actinidia arguta is significantly higher than male,the anthocyanin content can be used as convenient method to to identify the male and female plant of Actinidia arguta,both of trends and value of photosynthetic characteristics diurnal variation of male and female Actinidia arguta have a significant difference that is also an effective method to identify the male and female plant of Actinidia arguta;Peroxidase isoenzyme of male Actinidia arguta appears distinct band of sex-related that is the important evidence for selecting bisexual flower type plant;the genome of male and female Actinidia arguta has the obvious difference,meanwhile,based on sex-related SRAP can effectively distinguish the male and female plant.