The Abiotic Stress Tolerant Studies Of Maize MiR162 And MiR394 By Using Over-expression And STTM Technologies

Corn is an important source of food and industrial raw material,it has more than 470 years of cultural history in China.With the progress of industrial civilization,human activities make the environmental problems increasingly severe,such as drought,heat,salt and so on.These environmental problems seriously affected the cultivation of corn.So how to improve the corn stress tolerance is particularly important.With the increasing found number of microRNA in animals and plants,the microRNA functional studies are also urgently needed.In recent years,studies have shown that microRNAs involved not only in the growth and development of plants,but also played an important role in resistant to abiotic stresses.Since microRNA genes are small and often have overlapping genes,the traditional methods of gene mutation is difficult to achieve.Therefore,this study use the technology of STTM to silence microRNA,over-expression is also used to research the functions of microRNA from both directions.In our study,we find some candidate of maize abiotic stress related microRNAs from the database.Their over-expression vectors and short tandem target mimic(STTM)interference vectors constructed.Some of the constructions were transformed to the wild type Arabidopsis thaliana by means of inflorescence infection with Agrobacterium.The drought stress and salt stress tolerant scanning were conducted with transgenic lines miR162 O,miR162S,miR394 O and miR394 S.The results showed that increasing the over-expression of miR162 and miR394 can improve Arabidopsis salt tolerance,while reducing the expression of miR162 or increasing the expression of miR394 can improve drought Arabidopsis tolerance.