Design And Development Of Software System For Controlling A Portable On-Site Pathogen Detection Equipment

On-site rapid detection of infectious disease pathogens is extremely important for the timely prevention and diagnosis of large-scale infectious diseases,and there is an urgent need for miniaturized portable detection systems of pathogen nucleic acid.Based on the miniaturized integrated nucleic acid detection equipment which is independently developed by our research group,this paper designs and develops a software system that is easy to operate and functionally powerful,and can realize the automatic process of on-site infectious disease detection and Cloud storage.The research ideas and main work of this paper are as follows:(1)Platform developmentBased on the need of automatic control of the portable on-site pathogen detection equipment,the controlling requirements of the software system are analyzed,and the overall design of the software platform is proposed.According to the software demand,the whole system platform is divided into two parts: PC platform and Web platform.The PC platform is developed on Windows operation system with C#,which mainly realizes the automatic process of pathogen detection,experimental datum collection,analysis and management,and uploading test results to the web server.The Web platform is responsible for receiving infectious disease detection results,monitoring infectious disease outbreaks in real time,and presenting all statistical results of infectious disease data over web pages.(2)Datum AnalysisBased on the needs of on-site diagnosis,this paper designed an automated processing flow for real-time fluorescent PCR(q PCR)data collected by means of the PC platform,including datum preprocessing,Cq value calculation,and intelligent classification of PCR curves.In order to solve the shortcomings of traditional calculation method(Ct)which cannot accommodate datum noise and avoid datum outliers,this paper adopts a new Cq value calculation method(SDM,Cy0)and compares the similarities and differences of these different processing methods.The optimized real-time fluorescent PCR datum processing methods have improved the stability of this system and the feasibility,practicability and accuracy of the test results.At the same time,the intelligent classification of experimental results also provides users with a reference for optimizing experiments.(3)Experimental verificationThe software system developed in this paper was then applied on the on-site pathogen detection equipment.On one hand,the automated workflow of this software system is tested and verified by an infectious disease detection experiment.It is also compared with the traditional manual nucleic acid extraction experiment results;On the other hand,the verification of accuracy,stability and resolution of q PCR datum analysis methods designed by this paper and the practicality of Web online datum analysis are executed.The results show that the automatic detection of the on-site pathogen detection equipment under the control of the software system is correct and repeatable.Also,the datum analysis process is efficacious and robust,which can be well applied to our pathogen detection system.Lastly,the real-time warning function is proved to be fully available.