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Interference Based Quantitative Phase Imaging And Polarization Modulated Fluorescence Imaging

Posted on:2019-07-11Degree:MasterType:Thesis
Country:ChinaCandidate:C ZhengFull Text:PDF
GTID:2382330572961050Subject:Optical Engineering
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As an important observation tool in scientific research,optical microscopes are widely applied in Medical Science,Life Science and Material Science.According to the way the incident light interact with the sample under observation,optical microscopes can be divided into fluorescent imaging and non-fluorescent imaging.In fluorescent imaging,the sample under observation is fluorescent labelled and the emission after illumination excitation is detected for imaging.While in non-fluorescent imaging,the detected signal is mainly the transmitted light or scattered light after illumination.This dissertation first analyzed typical imaging methods in these two categories and proposed several novel methods according to their drawbacks.In non-fluorescent imaging,interference based quantitative phase imaging is capable of imaging transparent cells without labelling and conduct cell life cycle characterization.DPM stands out among these methods for its common-path geometry and real-time acquisition speed.However,the physical pinhole used in DPM requires high-precision alignment and regular drift correction.To solve this problem,we replaced the physical pinhole with a TN-LCD,together with a controlling program to realize automatic alignment of this digital pinhole.Moreover,we proposed a novel common-path QPI method based on DMD.Besides precise phase measurement,this method also provides a practical way for resolution enhancement in phase imaging.In fluorescent imaging,many techniques have made great efforts in resolution enhancement,such as STED,STORM and so on.Though the resolution improvement in these methods is tremendous,they are restricted by either the much complex optical system or high constraint on fluorescent dyes from wide applications.As a result,we proposed a super resolution method based on polarization modulation and sparse reconstruction.Till now,we have realized three dimensional super resolution both on confocal microscope and TIRF microscope.Since the only additional device to realize this technique is a simple polarization modulation module,we anticipate that the technique would be adopted with other microscopes with strong application prospect.
Keywords/Search Tags:quantitative phase imaging, super resolution microscopy, polarization modulation, image processing
PDF Full Text Request
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