Design And Experimental Research On The Controller Of High-voltage Steep Pulse Therapy Instrument

ObjectiveThe use of high-voltage Steep pulses to discharge biological tissues can produce nanoscale irreversible electroporation(IRE)effects on the cell membrane,leading to auto-apoptosis of biological tissues.Using this technique to ablate tumor cells,without heat damage,it can retain connective tissue such as blood vessels and nerve bundles near the tumor.This feature is ideal for treating tumors that cannot be operated on.MethodsBased on modern electronic technology,design the ablation devices.the voltage,pulse width,frequency and other parameters of output pulse are flexible and adjustable.To ensure accuracy and enhance its work safety and stability.The control system is applied to a high-voltage steep pulse therapeutic apparatus.The actual output of the therapeutic apparatus is tested in real-time online,and the steep pulse output accuracy and logic function of the system are tested.The output voltage is monitored in real time by a high-voltage probe,and the current flowing through the ablation tissue is detected by a current transformer.The live pig liver was used as the subject,ablation was performed at a dose of 1500 V/cm,50-100 ?s,and70 pulses.Postoperative life status and blood phase indicators of the test animals were observed.1 day and 3 days after surgery,respectively.At 7 days,14 days,and 21 days,the animals were dissected and analyzed for apoptosis and cell membrane electroporation at five time points to verify the efficacy of device ablation.Results1.This system realizes the generation of high-voltage steep pulse,and the pulse parameters are flexibly adjustable.The pulse voltage,pulse width,number of groups and other parameters can be set by the host computer.The fixed load test shows that the system output pulse voltage is within ±20V,the pulse width accuracy is within 1us,and the overcurrent protection alarming function can be realized through the feedback circuit and software control.2.In the observation period,no abnormal behavior was observed in the animals after the observation period.The results of HE staining showed that the sharp pulse ablation area had obvious boundaries.Obvious apoptosis was observed on the third day after surgery.Days and 21 days,significant tissue fibrosis and pseudolobular hyperplasia of liver tissue can be observed,and with the passage of time after surgery,inflammatory cell infiltration and tissue edema are gradually reduced.3.TEM electron microscopy showed that the large blood vessels and bile ducts in the ablation area remained intact.In the ablation area,macrophages and apoptotic bodies were observed,the cell membrane was incomplete,the boundary between cellswas not obvious,and vacuolation was serious.Nucleus pyknosis,with a small amount of inflammatory cell infiltration,etc.,In the late observation period,on the 14 th and21st days,the number of new cells increased,and the organization vacuolization gradually decreased.ConclusionThis research adopts DSP+CPLD control technology to realize the development of high-voltage steep pulse device,flexible pulse parameter control,stable and reliable operation and meeting the expected design requirements.Animal experiments show that there is no obvious abnormality in animals after surgery,and both HE staining and TEM electron microscopy show obvious Ablation of the tissue,and can completely retain the lumen of the great vessels,bile ducts and other structures.The steep pulse ablation device is shown to have a significant ablation effect and meet the requirements for clinical validation.