| Morchella esculenta is a long-established medicinal and edible homologous edible fungus.Wild morel is widely distributed in our country and has a wide variety.It is a world-renowned nourishment.With the continuous improvement of artificial cultivation technology,and the continuous progress of new countryside construction and modern agriculture,the production of Morchella esculenta has been greatly improved,but the main research on Morchella esculenta is currently focused on morel artificial cultivation technology and basic ingredients.There is a lack of sufficient research and development for proteins that account for about 25 % of Morchella esculenta content.In this study,extracting proteins from wild Morchella esculenta in Anze County,a characteristic resource in Shanxi Province,was used to study the optimal process for the preparation of antioxidant peptides by ultrasonic-assisted complex enzymatic hydrolysis.After multiple purifications,the peptide structural sequence was further identified,and the obtained peptide was sequenced.The in vivo antioxidant activity of the peptide component was investigated.The main research contents and results are as follows:(1)Analyze the basic constituents of wild Morchella esculenta and extract the components of Morchella esculenta with ultrasonic-assisted fractionation,of which albumin accounts for 54.10 % of the total protein content,followed by glutenin,which accounts for 11.34 %;globulin And gliadin were less,9.24 % and 5.13 %,respectively.The optimal process for extracting morel albumin by response surface method is: material-liquid ratio1:25(g/m L),extraction temperature 40 ?,extraction time 25 min.Under this optimized condition,morel albumin The yield was stable at 15.11 % ±0.14 %.(2)Determination of amino acids in Morchella esculenta protein,the results show that the content of essential amino acids in Morchella esculenta protein is rich,accounting for about 40 % of the total amino acid content,ofwhich umami amino acids that play a role of tender taste account for25.91 %,While sweet amino acids accounted for 23.56 %.By comparing the anti-oxidant capacity of crude protein and albumin of Morchella esculenta spp.,It was found that albumin has superior antioxidant properties.At a concentration of 1.2 mg/m L,the DPPH radical and ·OH radical scavenging rates were 77.13 % and 64.52 %,respectively.(3)The crude protein of morel was used as a substrate,and a complex enzyme composed of alkaline protease and trypsin(enzyme activity ratio of7: 3)was used for enzymolysis,and the anti-oxidative peptidase was carried out with the response rate of DPPH free radicals The optimization of the hydrolysis process yields the best enzymatic hydrolysis process: the enzymatic hydrolysis time is 60 min,the enzymatic hydrolysis temperature is55.69 ?,the enzymatic hydrolysis p H is 7.95,the amount of enzyme added is 4000 U/g,and the DPPH free radical scavenging rate of the antioxidant peptide obtained by the enzymatic hydrolysis.It is 89.09 ± 0.21 %.(4)The peptide obtained by enzymolysis was separated and purified by ultrafiltration,DEAE-32,and Sephadex G-25.Finally,the component with the highest antioxidant capacity was selected,and its sequence was identified as FPLIPGH by mass spectrometry.This component peptide was subjected to in vivo antioxidant evaluation.D-galactose was used to model aging mice.After continuous intragastric administration of the mice for one month,The liver group GSH-Px,SOD,and CAT activity in the experimental group was significantly increased compared with the model group,and the MDA content was significantly reduced,indicating that the morel G2 component has good antioxidant properties in mice. |
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