Study On The Synthesis And Control Ice Crystals Performance Of Materials Inspired By Antifreeze Protein

In nature,antifreeze proteins(AFPs)present in plants,fish,and insects living in cold regions exhibit unique abilities to decrease the freezing temperature of water,inhibit ice growth and recrystallization.Because the order of hydroxyl groups chemical structures of AFPs,it can match with the ordered oxygen atoms of the ice and adsorb on the surface of ice crystal generating microcurvatures on the ice.The preferred adsorption on the ice crystal also provides these AFPs with an ice shaping effect as well as inhibiting the ice growth and ice recrystallization.AFPs are the natural function materials with anti-freeze activity.However,AFPs suffer from difficult extraction,low stability,which limit wide application in cryopreservation.Thus,AFP-inspired cryoprotectants with low cytotoxicity,simple design,and large-scale production are highly desired.In this thesis,a simple method was used to prepare the carbon dots and Janus inspired by antifreeze protein and study on the control ice crystals performance of carbon dots and Janus inspired by antifreeze protein.The main content is follow:(1)Herein,carbon dots from glucose(G-CDs)were synthesized by hydrothermal carbonization.The G-CDs is basically consistent with the functional characteristics of AFP.The preferred adsorption on ice crystals provides the G-CDs with a specific ice shaping effect while inhibiting ice growth and recrystallization.The high inhibition of ice recrystallization(IRI)activity and low cytotoxicity of the G-CDs make them promising cryoprotectant materials.Thus,G-CDs without any organic solvent can significantly improve the sheep red blood cells(RBCs)recovery to ca.60 %.The use of glucose as a starting material provides these G-CDs with low-cost,biocompatibility,commercially availability,and mass-production characteristics,providing new ideas and important experimental basis for the synthesis of both cryopreservation materials CDs from sugars.(2)With glucose as the precursor,G-CDs with different particle sizes could be prepared by controlling the concentration and temperature of the precursors.G-CDs with different particle sizes were characterized using a nanoliter osmometer and cold stage with precise temperature control device.It is concluded that small-sized G-CDs have a greater impact on the morphology of ice crystals,and have a stronger ability to suppress ice growth and ice recrystallization.(3)Herein,the ligand competition method was used to prepare S-Au Janus.Then the 3-S-Au Janus was prepared by adding Sodium 3-mercapto-1-propanesulfonate.S-Au Janus and 3-S-Au Janus were characterized using a nanoliter osmometer and cold stage with precise temperature control device.It is concluded that the G-CDs with a specific ice shaping effect while inhibiting ice growth and recrystallization.