Nonlinear Analysis And Steady-state Optimization Of Continuous Dissimilation Process Of Glycerol In Two-stage Culture

In bio-dissimilation process of glycerol to 1,3-propanediol,industrial by-product glycerol is used as fermentation substrate.This method has advantages such as mild reaction conditions and high commercial development value,so it has become a research hotspot.Researching the nonlinear analysis and process optimization of bio-dissimilation process of glycerol to 1,3-propanediol mathematically,is of great significance to guide the actual production process of 1,3-propanediol and improve the production technology.In this paper,aiming at the continuous dissimilation process of glycerol to1,3-propanediol in two-stage culture,some study of nonlinear analysis and steady-state optimization is done.The main contents and main results of this paper are as follows:1.Aiming at the continuous dissimilation process of glycerol to 1,3-propanediol in two-stage culture,the calculation method of system equilibrium points and the analytical results,such as distribution of equilibrium points,distribution of the number of equilibrium groups and the stability of equilibrium points,are given.2.Aiming at the continuous dissimilation process of glycerol to 1,3-propanediol in two-stage culture,a steady-state optimization model,with the maximum yield of1,3-propanediol as optimization objective,the steady-state operation and bounded variables as constraints,is established.And the calculation results show that,compared with the existing literature,this method not only avoids the "wash out" phenomenon which is easy to occur in microbiological fermentation,but also obtains the optimization result of larger yield and less residual substrate glycerol concentration than one-stage culture.3.Aiming at the continuous dissimilation process of glycerol to 1,3-propanediol in two-stage culture,with comprehensively considering the requirements of steady-stateoperating conditions,bounded variables and stability,a steady-state optimization model is established to maximize the yield of the main product 1,3-propanediol.Then an iterative algorithm framework is constructed to solve the model.The calculation results show that,the method of this paper can not only stabilize the system,but also maximize the yield of 1,3-propanediol.