The Preparation And Evaluation Of Astaxanthin Micro-Nanocarriers

Astaxanthin is a non-vitamin A source of fat-soluble ketone carotenoids.Its special conjugated double bonds and hydroxyl groups in its molecular structure enable it to have super antioxidant activity.So far,Astaxanthin is the most powerful antioxidant in nature.Astaxanthin has many physiological functions,such as anti-aging,anti-tumor,anti-inflammatory,preventing cardiovascular and cerebrovascular diseases,treatment of diabetes,and improvement of motor function,etc.It has considerable practical value and application prospect in food,health care products and medicine industry.However,its special structure makes it have the application weakness of low bioavailability and extremely oxidation.In this paper,we use the method of micro-nano technology to improve the weakness.Astaxanthin solid emulsion drug delivery system(ASX-SEDDS)was prepared by physical mixed adsorption method.Particle sizes obtained by rehydration were between 170-180 nm,and PdI was below 0.3.The results of dissolution in vitro showed that the carrier could significantly improve the solubility of astaxanthin and had a significant sustained release effect.By comparing the difference between two solid adsorption carriers,it can be seen that anhydrous calcium hydrophosphate has better sustained release effect than silicon dioxide,and the adsorbed self-emulsion also has better long-term stability.So anhydrous calcium hydrophosphate is an ideal carrier.To explores the effect of different protectant adding to the ASX-SEDDS,we chose two different protective-soybean lecithin powder and sodium caseinate,probe into whether it affect the physical and chemical properties of astaxanthin or not,the results shows that the thermodynamic stability,the character when it dissolving into the water and in vitro dissolution and digestive properties have no obvious change.The stability of ASX-SEDDS was significantly improved by adding the protectant.Compared with the two protectants,it can be seen that the soybean powder phospholipids have better protective effect.Astaxanthin nanostructured lipid carrier(ASX-NLC)was prepared by high pressure homogenization,and its microstructure,storage stability,in vitro simulated release and in vitro digestion were studied by us.The particle size was 145.30±1.25 nm,and the PdI was 0.27 ±0.06.Nanoparticles have good dispersion in aqueous solution,and can maintain a relatively stable state and a high astaxanthin retention rate during the storage period of 25 oC after 28 days.Encapsulated astaxanthin has better long-term chemical stability than unencapsulated astaxanthin,and its simulated release in vitro is a slow and continuous process.The preliminary study on the application layer of the prepared astaxanthin micro-nano carrier was conducted,and astaxanthin solid tea and astaxanthin milk mixture were prepared.The results showed that astaxanthin could be stable in both,which contributed to the application of astaxanthin in the field of functional food.We developed two different astaxanthin micro-nano carriers in this paper to provide new ideas for solving the bottleneck of astaxanthin application,and provide more space for the application of astaxanthin in the field of functional food.