Fabrication Of Ovalbumin Based Complex Carrier And Their Ability To Encapsulate Kaempferol/Tannic Acid

Natural active ingredients not only have the ability of scavenging free radicals in the body,but also maintain body health,physical activity,growth and development.However,there are problems such as low bioavailability,rapid metabolism,poor solubility,and rapid systemic elimination.Natural polymer materials constructed by self-assembly have many potential and feasible advantages and values for delivery vehicles such as environmental friendliness,modifiability,strong protection,small size,and easy penetration.It is the research focus of the current polyphenol drug delivery system to confer efficient and targeted controlled release of carriers.In this paper,ovalbumin（OVA）was used as the basic material to prepare ovalbumin nanoparticles by self-assembly,and then used sodium alginate（SA）to prepare ovalbumin-sodium alginate composite carrier.Then protected and encapsulated the hydrophobic drug kaempferol（KAE）and the hydrophilic drug tannic acid（TA）through the carrier.In order to achieve the purpose of targeted delivery,the TA-Fe3+coating film with pH-responsive drug release was prepared by using the characteristics of the tannic acid and metal ion to form a three-dimensional network coating film.The main conclusions of this article were as follows:1.Kaempferol/tannic acid were well encapsulated in ovalbumin nanoparticles.Ovalbumin binded well with hydrophilic tannic acid,the protein structure had the highest stability,and the protein maintained a stable three-dimensional structure;The loading of kaempferol improved the thermal stability of ovalbumin loaded with tannic acid in composite particles;Hydrophilic tannic acid weakened the conformational change of ovalbumin loaded with kaempferol composite particles,and enhanced the interaction between ovalbumin and hydrophobic polyphenol kaempferol.The composite particles loaded with two polyphenols simultaneously had the strongest system stability and antioxidant activity.The three types of composite particles were uniformly dispersed and uniform in size.2.Kaempferol and tannic acid were encapsulated in ovalbumin-sodium alginate nanoparticles in an amorphous state,and the good encapsulation effect was enhanced by OVA-KAE-SA>OVA-TA-KAE-SA>OVA-TA-SA in turn.The content of encapsulated polyphenols in OVA-SA composite particles was 2.7³.2 times that of OVA nanoparticles.After OVA-SA nanoparticles were loaded with KAE/TA than single OVA nanoparticles,the protein conformation changed little and the effective particle size was small.OVA-TA-KAE-SA’s highest thermal denaturation center temperature may be due to the synergy of the thermal stability of the two polyphenols.OVA-TA-SA composite particles had the strongest antioxidant activity and antibacterial ability,indicating that the hydrophilic TA enhancement effect was stronger than the hydrophobic KAE.3.The appearance of small diffraction peaks in the carrier proved the formation of the TA/Fe³⁺coating on the surface of the composite particles.The conformation of the composite particles coated with TA/Fe³⁺changed.The coating of TA/Fe³⁺increased the system stability and thermal stability of the composite particles,and the carrier was also endowed with antioxidant activity.The compound with the strongest antioxidant activity was the pHOVA-TA-KAE-SA composite particle,which had the same antibacterial effect.