Preparation And Properties Of Ferrous Chelate Of Fish Scale Collagen Peptide

Iron is an important trace element in the human body.It participates in a variety of life activities such as oxygen metabolism,energy metabolism,mitochondrial electron transfer,muscle function and hematopoiesis.How to improve the absorption and utilization of iron in the human body has become the focus of scholars' research..Polypeptide ferrous chelate is a new substance produced by the chelation reaction between peptide and ferrous ion,and can be used as a new type of iron supplement.Tilapia is one of the important species of freshwater aquaculture in China.It is widely consumed in our country and has a large consumption.It has important economic and edible values.A large amount of scraps have not been obtained during the processing of tilapia.Utilization has severely limited its further development.Fish gelatin is a traditional gelatin substitute with great potential and has become a research hotspot in recent years.Compared with traditional gelatin,fish gelatin has the characteristics of wide source,low price,and high nutritional value,and fish gelatin has no religious and health restrictions,which meets the needs of special populations.In this study,tilapia fish scale gelatin was used as a raw material to prepare collagen peptides,and the single factor and response surface method were used to optimize the peptide-ferrous chelation conditions to obtain the optimal preparation process of the peptide ferrous chelate.,Fourier infrared spectroscopy,X-ray diffraction and amino acid analyzer to analyze the structure and amino acid composition of the chelate;and to investigate the stability of the chelate through in vitro simulated digestion tests,and finally obtain a peptide ferrous chelate product and its preparation The technology can promote the development of dietary iron supplements and the deep processing and highvalue utilization of tilapia fish scales.The main research conclusions are summarized as follows:(1)Using tilapia scale gelatin as the raw material,configure a 5% gelatin solution,place it in a 50? constant temperature water bath for 15 minutes,adjust the pH to 8 with 0.1 mol / L NaOH,and add gelatin with a mass ratio of 1%.Alkaline protease was bathed in water for 30 min,pH was adjusted to 8 again,and hydrolysis was 4.5 h.After the hydrolysis was completed,the water was inactivated by boiling in a water bath for 10 min.After cooling,centrifuged at 4000 r / min for 15 min to obtain the supernatant.(2)With the chelate yield and chelation rate as indicators,the preparation process of the peptide ferrous chelate was optimized by single factor and response surface methodology.The results of single-factor experiments showed that the chelation reaction was greatly affected by three factors: pH,the concentration of the peptide solution,and the mass ratio of the peptide to the ferrous salt.The response surface software was used to design and verify the experiment.The reaction conditions were pH 5.30,peptide concentration 3.00%,and peptide to ferrous salt mass ratio 3.2: 1.Under these conditions,the chelation rate was 82% and the chelate yield was 65.43%.(3)The structure and amino acid composition of the chelate were analyzed by scanning electron microscopy,Fourier infrared spectroscopy,X-ray diffraction,and amino acid analyzer.The results showed that the amino acid composition conformed to the composition characteristics of collagen peptides.Aspartate,glutamic acid,and lysine were chelated.The content of acid,histidine and arginine increased.Scanning electron microscopy,infrared spectroscopy,and X-ray diffraction results showed that the peptide and ferrous ion combined with the coordination bond to form the peptide ferrous chelate.(4)The stability and biological toxicity of chelate were evaluated by in vitro simulated digestion and cell experiments.Digestive test results of simulated gastric juice and intestinal juice showed that the peptide chelate was stable under the action of pepsin and trypsin.Caco-2 cytotoxicity test showed that low concentration of peptide ferrous chelate can significantly promote cell production,which laid a theoretical foundation for further research on the mechanism of peptide ferrous chelate on cells.