Study On Antifouling Properties Of Glass Microstructured Surface Modified By Bio-peptide

With the vigorous development of the global shipping industry,the loss of marine bio-fouling has caused more and more losses to various marine activities.The use of traditional chemical antifouling coatings to prevent fouling organisms can seriously affect the balance of the marine ecosystem and is contrary to today's green environmental themes.There is an urgent need to find an environmentally friendly anti-fouling technology.A single anti-fouling method is not ideal for the antifouling effect of a wide variety of marine fouling organisms.Studies on sharks have shown that their anti-fouling properties are synergistically achieved by the dense microstructure on the epidermis and the mucus secreting anti-protein adsorption.This paper imitates the anti-fouling mode of sharks,uses etching processing technology to process microstructures on common glass surfaces,and then uses bio-peptides to modify the microstructure surface to analyze the physical and chemical properties and anti-fouling properties of the material surface.The synergistic effect of biological peptides and surface microstructures on the prevention of fouling organisms.Firstly,according to the attachment point theory and other existing surface microstructure anti-fouling theories,the shape and size of the microstructure are designed,and three types of microstructures are designed.Each type of structure has three depths(the processing depth is 3,5,7 ?m),a total of 9 different surface microstructures.The surface of the processed microstructure was observed using a laser scanning confocal microscopy.The structural dimensional error was less than 10%,and the processed microstructure surface met the design requirements.The surface of the glass sample on which the microstructure has been processed is modified using nisin.The bio-peptide was modified to the surface of the glass sample using dopamine as a coupling agent.The modified surface was analyzed using an X-ray photoelectron spectrometer.Comparing the change of the ratio of the five elements of C,N,Si,O and S on the surface of the smooth surface,dopamine-modified surface and the bio-peptide modification,the S element appeared only on the surface of the bio-peptide modification,indicating that the bio-peptide was successfully bound to the surface of the sample.The contact angle measuring instrument was used to measure the static water contact angle of the material surface.The original surface contact angle was 39.3°.After the surface microstructure was processed,the contact angle increased,and the maximum contact angle was 82.0°.The surface contact angle after bio-peptide treatment ranged from 13° to 25°,compared with the same type of microstructure surface contact angle has a significant decline.The bacterial biofilm test and the adhesion test of algae showed that the selected nisin had a certain inhibitory effect on the fouling organism.The inhibitory effect of surface microstructure on fouling organisms depends on their relative size to the fouling organism.For bacteria,the size is much smaller than the microstructure size,can be embedded in the groove,and is protected,so the microstructure designed in this paper will increase the adhesion of bacteria;for algae,the size is slightly larger than the microstructure size.It can only be placed on the surface of the microstructured convex surface,and the adhesion strength is low,and it is easy to be washed away,so the microstructure can effectively reduce the adhesion of the algae.At the same time,as the depth of the microstructure increases,the aggravating effect of bacterial adhesion and the inhibition effect of algae adhesion are enhanced.